Immunomodulating red blood cell coating for mitigation of foreign body reactionsOA
Chronic inflammation of biomedical implants usually leads to fibrosis and device malfunction in the long term.To address these issues,a cell-crosslinked coating of red blood cells(RBCs)was developed to imitate a self-friendly biological membrane and camouflage the implants from immune system.Using the widely applied poly(dimethylsiloxane)(PDMS)as a model substrate,a natural polymer hyaluronic acid(HA)layer was constructed upon PDMS(PDMS-HA),which was further decorated with RBCs(PDMS-HA-RBC).Compared to pristine PDMS,both PDMS-HA and PDMS-HA-RBC notably polarized the original macrophages into an anti-inflammatory phenotype(M2)rather than a pro-inflammatory phenotype(M1).Especially,PDMS-HA-RBC exhibited the highest M2/M1 ratios of macrophages,suggesting efficient modulation effects of inflammation reactions by the RBCs coating.Moreover,in vivo results found that PDMS induced considerable foreign body reactions(FBRs)and extensive fibrosis formation.In contrast,PDMS-HA revealed a significantly thinner fibrotic layer while PDMS-HA-RBC induced the least amount of fibrosis.In addition,PDMS-HA-RBC exhibited the highest fluorescent intensity of CD206(M2 antigen)and the lowest fluorescent intensity of CD86(M1 antigen).It was speculated that the RBCs coating-mediated macrophage polarization was mainly attributed to the presence of immune escape antigens(such as CD47 and CD59)upon the cell coating.Altogether,our living RBCs coating demonstrated significant potentials in mitigating FBRs of PDMS,indicating their promising applications in surface engineering of various biomedical implants.
Yi Ye;Jinyun Wu;Tao Sun;Minghong Huang;Jiaping Pan;Danjing Hu;Keying Chen;Dawei Wang;Chenxi Wang;Yangkun Shen;Qi Chen;Baolin Huang
Joint Research Centre on Medicine,Xiangshan Hospital of Wenzhou Medical University,Ningbo,Zhejiang 315700,PR ChinaJoint Research Centre on Medicine,Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,Zhejiang 325000,PR China Fujian Key Laboratory of Innate Immune Biology,Biomedical Research Center of South China,College of Life Science,Fujian Normal University,Fuzhou,Fujian 350117,PR ChinaJoint Research Centre on Medicine,Xiangshan Hospital of Wenzhou Medical University,Ningbo,Zhejiang 315700,PR ChinaJoint Research Centre on Medicine,Xiangshan Hospital of Wenzhou Medical University,Ningbo,Zhejiang 315700,PR ChinaJoint Research Centre on Medicine,Xiangshan Hospital of Wenzhou Medical University,Ningbo,Zhejiang 315700,PR ChinaJoint Research Centre on Medicine,Xiangshan Hospital of Wenzhou Medical University,Ningbo,Zhejiang 315700,PR ChinaJoint Research Centre on Medicine,Xiangshan Hospital of Wenzhou Medical University,Ningbo,Zhejiang 315700,PR ChinaFujian Key Laboratory of Innate Immune Biology,Biomedical Research Center of South China,College of Life Science,Fujian Normal University,Fuzhou,Fujian 350117,PR ChinaFujian Key Laboratory of Innate Immune Biology,Biomedical Research Center of South China,College of Life Science,Fujian Normal University,Fuzhou,Fujian 350117,PR ChinaFujian Key Laboratory of Innate Immune Biology,Biomedical Research Center of South China,College of Life Science,Fujian Normal University,Fuzhou,Fujian 350117,PR ChinaFujian Key Laboratory of Innate Immune Biology,Biomedical Research Center of South China,College of Life Science,Fujian Normal University,Fuzhou,Fujian 350117,PR ChinaJoint Research Centre on Medicine,Xiangshan Hospital of Wenzhou Medical University,Ningbo,Zhejiang 315700,PR China Joint Research Centre on Medicine,Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,Zhejiang 325000,PR China Fujian Key Laboratory of Innate Immune Biology,Biomedical Research Center of South China,College of Life Science,Fujian Normal University,Fuzhou,Fujian 350117,PR China
医药卫生
PDMSCell coatingForeign body reactionBiomedical implant
《Engineered Regeneration》 2025 (1)
P.218-229,12
supported by the Joint Research Centre on Medicine,Wenzhou Institute,University of Chinese Academy of Sciences and Xiangshan Hospital of Wenzhou Medical University(Grant No:XSZD2024009)the startup grants from Wenzhou Institute,University of Chinese Academy of Sciences(Grant No:WIUCASQD2024005)the National Natural Science Foundation of China(Grant No.82350126 and U22A20326)the Innovation of Science and Technology,Fujian Province(Grant No.2023Y9342 and 2023Y9431).
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