p53、CyclinD1和MDM2在浸润性乳腺癌中的表达及临床意义OA

Objective To investigate the expression patterns and clinical significance of p53,the cell cycle protein D1(CyclinD1),and murine double minute 2(MDM2)in invasive breast carcinoma(IBC).Methods A total of 111 paraffin-embedded primary IBC tissue specimens surgically resected between March 2015 and February 2017 at the First Affiliated Hospital of Wenzhou Medical University were collected,along with 64 matched adjacent non-tumorous breast tissue specimens(47 cases had no paired adjacent tissues).Detailed clinicopathological data were recorded.Tissue microarray(TMA)and immunohistochemistry(IHC)were employed to detect the protein expression levels of p53,CyclinD1,and MDM2 in cancer tissues and corresponding adjacent tissues.The correlations between the three proteins and clinicopathological features,as well as patient prognosis,were analyzed.Results The positive expression rates of p53,CyclinD1,and MDM2 in IBC tissues were significantly higher than those in adjacent tissues(all P＜0.05).The positive expression rate of p53 in IBC tissues of patients with TNM stage Ⅱ-Ⅳ,histological grade Ⅱ-Ⅲ,lymphovascular invasion(LVI),and lymph node metastasis was significantly higher than those with TNM stage Ⅰ-Ⅱ,histological grade Ⅰ,no LVI,and no lymph node metastasis(all P＜0.05).The positive rate of CyclinD1 in IBC tissues of patients without lymph node metastasis and those with positive estrogen receptor(ER)or progesterone receptor(PR)was significantly higher when compared with those with lymph node metastasis,and negative ER and PR(all P＜0.05).MDM2 positive expression in IBC tissues of ER-positive and PR-positive patients was significantly higher than those of ER-negative and PR-negative patients(both P＜0.05).Cox regression analysis indicated that positive MDM2 expression,and negative CyclinD1 expression in IBC tissues were independent risk factors for failing to achieve 3-year disease-free survival(DFS)(all P＜0.05).Conclusion The expressions of p53,CyclinD1,and MDM2 are up-regulated in IBC tissues.Elevated expression of MDM2 is associated with poor prognosis of patients with IBC,whereas up-regulated CyclinD1 expression serves as a protective factor for a favorable prognosis.