山芝麻化学成分研究OA

Objective:To study the chemical constituents of Helicteres angustifolia.Methods:The ethyl acetate fraction of methanol extract from the root of Helicteres angustifolia was isolated and purified by using chromatographic techniques such as silica gel,ODS,Sephadex LH-20 glucan gel,and preparative HPLC,and the compound structures were identified by modern spectroscopy.Results:Thir-ty-two compounds were isolated from Helicteres angustifolia and identified as(2R,8S,9R,10R,13R,14S,16R,17R)-17-[(R,E)-2,6-dihydroxy-6-methyl-3-oxohept-4-en-2-yl]-2,16-dihydroxy-4,4,9,13,14-pentamethyl-1,4,7,8,9,10,12,13,14,15,16,17-dodeca-hydro-3H-cyclopenta[a]phenanthrene-3,11(2H)-dione(1),cucurbitacin I(2),cucurbitacin E(3),cucurbitacin J(4),hexanorcucurb-itacin I(5),oleanolic acid 3β-caffeate(6),3β-acetoxy-27-(benzoyloxy)olean-12-en-28-oic acid methyl ester(7),3β-acetoxy-27-trans-caffeoyloxyolean-12-en-28-oic acid methyl ester(8),3β-(p-hydroxy-trans-cinnamoyloxy)olean-12-en-28-oic acid(9),3β-hydraxyolean-12-en-27-benzoyloxy-28-oate(10),3β-hydroxyoleana-11,13(18)-dien-28-oic acid(11),3β-acetoxy-27-[(4-hydroxybenzoyl)oxy]olean-12-en-28-oic acid methyl ester(12),betulic acid(13),pyracrenic acid(14),methyl helicterate(15),6,7,9α-trihydroxy-3,8,11α-trimethylcyclohexo-[d,e]-coumarin(16),6,7-dihydroxy-3,8,11-trimethylcyclohexo-[d,e]-coumarin(17),mansorin I(18),man-sonone F(19),mansonone E(20),mansonone H(21),mansonone M(22),syringaresinol(23),dihydrodehydroconiferyl alcohol(24),buddlenol E(25),4-hydroxybenzoic acid(26),ferulyl aldehyde(27),methyl(E)-octadec-9-enoate(28),palmitic acid(29),β-sitoster-ol(30),stigmasterol(31),hibiscone C(32),respectively.Conclusion:Among them,compound 1 is a new configuration,and compounds 6,8,18,25 to 29 and 32 are isolated from Helicteres angustifolia for the first time.