特异性PCR法鉴别白僵菌及其混伪品OA

Objective:To establish a specific polymerase chain reaction(PCR)method for the rapid identification of Bombyx Ba-tryticatus and its adulterants.Methods:The ITS sequence of Beauveria bassiana,a fungal species associated with Bombyx Batryticatus,was compared with the corresponding gene sequences of related species.The specific primer TF2/TR2 was designed based on the frag-ments with a high number variation sites,and the PCR reaction conditions were optimized and validated.Results:At an annealing tem-perature of 64℃and with 30 cycles,all 14 batches of Bombyx Batryticatus,the powdered medicinal materials containing Bombyx Ba-tryticatus,and standard samples of Beauveria bassiana yielded a single clear target band at 258 bp.In contrast,none of the 27 batches of adulterants displayed this band.Conclusion:The specific PCR method established in this research institute accurately realizes the au-thenticity identification of Bombyx Batryticatus and its adulterants.This method has good specificity,is convenient and fast to operate,and is suitable for the quality control of Bombyx Batryticatus,providing technical support for addressing the issues of the stability and safety of Bombyx Batryticatus medicinal material.