珊瑚七十味丸经口给予小鼠和大鼠的急性毒性研究OA

Objective:To study the acute toxicity of Shanhu Qishiwei Pills,and to provide reference for the dose design of long-term toxicity test and clinical safe medication.Methods:Mice and rats in the three groups with the maximum dosages of Shanhu Qishiwei Pills were administered the medicinal solution of Shanhu Qishiwei Pills via gavage once,twice,and three times respectively within 24 hours,with an 8-hour interval between each administration.Mice were given the medicinal solution of Shanhu Qishiwei Pills at a concentration of 0.2 g·mL-1 at a dose of 40 mL·kg-1 each time,while rats were administered the medicinal solution at a concentration of 0.25 g·mL-1 at a dose of 20 mL·kg-1 each time.Subsequently,the animals were observed for toxic reactions over a 14-day period.Results:No animal death occurred during the observation period.Although Shanhu Qishiwei Pills exert an influence on the body weight of rats,the affected rats can all recover their original weight.Shanhu Qishiwei Pills might have effects on the hematological and biochemical indicators of experimental animals at the maximum administered dose.The main manifestations included an increase in total protein concentration(TP),a decrease in blood glucose(Glu)and mean corpuscular hemoglobin concentration(MCHC),as well as changes in liver-related indicators such as alanine aminotransferase(ALT)and total bilirubin(TBIL).It was noteworthy that the specific direction and extent of these changes exhibited certain species and gender differences.Female rats exhibited increased RBC,TBIL,and TP levels.Male rats showed decreased MCHC levels but increased LymPh and Glu levels.Conclusion:The maximum oral administration doses of Shanhu Qishiwei Pills to mice and rats by oral administration are 24 g·kg-1 and 15 g·kg-1,respectively,which are 1 440 times and 900 times of the daily dose for clinical adults.Therefore,the safety range is relatively wide.However,Shanhu Qishiwei Pills have certain effects on the body weight,feed consumption,hematology.Therefore,close monitoring should be conducted during clinical application.