乌头茎叶总生物碱提取纯化工艺研究OA

Objective:The aim of this experiment was to optimize the extraction and purification process conditions of total alkaloids from the stem leaves of Aconitum carmichaelii Debx..Methods:The effects of hydrochloric acid concentration,materi-al-liquid ratio,extraction temperature,extraction time and number of extractions on the extraction process of total alkaloids from the stem leaves of Aconitum carmichaelii Debx.were investigated by using a one-way experiment with the extraction rate of total alkaloids as the evaluation index.Based on the one-way results,the optimal extraction process of total alkaloids from the stem and leaves of Aconitum carmichaelii Debx.was optimized using the L9(34)orthogonal test method.After this,the total alkaloids were purified by enrichment using cation exchange lipids.The adsorption and desorption rates of alkaloids were used as indicators to find the optimal purification process,respectively.Results:The best extraction process could be determined as hydrochloric acid concentration of 0.1 mol·L-1,material-liquid ratio of 1:20,extraction temperature of 70℃,extraction time of 3 h,and the number of extraction times of two.Under these conditions,the average extraction rate of total alkaloids from the stem and leaves of Aco-nitum carmichaelii Debx.was 0.78%.The best purification process was to select 001×12 type cation exchange resin.The concen-tration of the sample solution was 0.3 g of the original herb per 1 mL and its pH was 3.The volume of the sample was 0.5 BV,and 3 BV of ultrapure water was used to remove the impurities,and then the sample was eluted with 4 BV of 3 mol·L-1 ammonia-95%ethanol.Conclusion:The extraction and purification process preferred in this experiment is stable and reliable,and lays the foun-dation for the subsequent research and development of this herb.