开心散基准样品多指标成分的薄层色谱鉴定OA

Objective:To establish a thin layer chromatography(TLC)method for the efficient identification of multiple index com-ponents in the reference samples of the classic prescription"Kaixinsan".Methods:This study systematically investigated the effects of major factors such as the preparation of the test sample,the composition of the developing agent,the developing environment(tempera-ture and humidity),and thin-layer chromatography plates from different manufacturers on the thin-layer chromatography behavior of six indicator components,namely ginsenoside Rb1,ginsenoside Re,tenuifolin,β-asarone,dehydrotumulosic acid and pachymic acid,in the Kaixinsan reference sample.And it was verified through actual measurement of samples.Results:The established thin-layer chromatogra-phy method effectively isolated and identified six indicator components in Kaixinsan.The spots of the six indicator components were clearly colored,with good separation,high reproducibility,negative without interference,and good durability.Conclusion:The thin-layer chromatography identification method for simultaneous detection of multiple standard components in the Kaixinsan reference sample es-tablished by this research institute has strong specificity and applicability,providing necessary experimental basis for the subsequent re-search and formulation of Kaixinsan quality standards.