维西糯山药遗传多样性分析及指纹编码构建OA

Objective:To determine the genetic relationship between Dioscorea opposita from Weixi Lisu Autonomous County and other germplasms,to construct the DNA fingerprint map and QR code of Dioscorea opposita from Weixi Lisu Autonomous County,so as to provide a scientific basis for protecting the characteristic varieties of Dioscorea opposita from Weixi Lisu Autonomous County.Meth-ods:By using the SSR molecular marker method,the DNA samples of 40 Dioscorea opposita germplasms were amplified to analyze the genetic diversity characteristics.The DNA fingerprint map and QR code of the germplasm resources of Dioscorea opposita from Weixi Lisu Autonomous County were constructed by using software such as NTSYS2.0e and Excel.Results:Among the 23 pairs of candidate SSR primers,6 pairs of amplitation-stable SSR primers were finally selected.The genetic diversity of Dioscorea opposita from Weixi Lisu Autonomous County was obvious.Among them,the variation of the Dioscorea opposita from Weixi Lisu Autonomous County grown in Kangpu Township was particularly prominent.The results of cluster analysis showed that when the similarity coefficient was 0.28,40 Di-oscorea opposita germplasm resources could be classified into three major categories.Conclusion:The Dioscorea opposita from Weixi Lisu Autonomous County in Baohe Town and Baijixun Township has been recognized as a major high-quality cultivated variety.Based on this,this study constructs the DNA fingerprint map and QR code of Dioscorea opposita from Weixi Lisu Autonomous County,laying a theoretical basis and technical support for the identification of Dioscorea opposita from Weixi Lisu Autonomous County germplasm re-sources and the breeding of superior varieties.