应用环介导等温扩增技术鉴别毛慈菇与冰球子OA

Objective:To establish a specific loop-mediated isothermal amplification(LAMP)method system for the rapid identifi-cation of two Chinese herbal medicines,Cremastra appendiculata and Pleione bulbocodioides.Methods:The rbcL coding region fragment in the chloroplast fragment was selected as the detection target.Specific primers for the identification of Cremastra appendiculata and Pleione bulbocodioides were designed.The LAMP identification method was established,and its methodological indicators such as speci-ficity,sensitivity and applicability were investigated.Results:The established LAMP rapid detection method under the reaction tempera-ture of 65℃and reaction time of 60 min could effectively distinguish the two easily-confused Chinese medicinal materials Cremastra appendiculata and Pleione bulbocodioides.The detection sensitivity could reach 500 ag/μL,and the reaction results were easy to ob-serve.Conclusion:The LAMP rapid identification method for the Chinese medicinal materials Cremastra appendiculata and Pleione bul-bocodioides established in this study has the characteristics of high sensitivity,good specificity,rapidity and practicality,and easy promo-tion.It is suitable for the quality monitoring of two Chinese medicinal materials Cremastra appendiculata and Pleione bulbocodioides.