苦参碱通过HMGB1/RAGE信号通路抑制人乳头瘤病毒阳性宫颈癌的发生和发展OA

Objective To investigate the effects of matrine on the occurrence and development of human papilloma virus(HPV)-positive cervical cancer via regulation of the HMGB1/RAGE signaling pathway.Methods HeLa cells were randomly divided into control,matrine,pc-HMGB1,pc-NC,and matrine+pc-HMGB1 groups.CCK-8 and flow cytometry were used to detect cell viability and apoptosis.Transwell and cell scratch assays were performed to assess cell invasion and matrine expression,respectively.Three-di-mensional in vitro cultures were used to detect vascular mimicry(VM)in cells.Western blotting was used to detect the expression of HMGB1/RAGE signaling pathway proteins in the cells.We constructed HeLa xenograft nude mice and examined the effects of matrine on tumor growth,angiogenesis,and the expression of HMGB1/RAGE signaling pathway proteins.Results The cell viability,invasion number,matrine rate,lumen formation number,HMGB1 and RAGE protein expression in the matrine group were lower than those in the control group,and the apoptosis rate was increased(P<0.05).The trend of changes in various indicators in the pc-HMGB1 group was the opposite of that in the matrine group;the nude mouse experiment further confirmed matrine's inhibitory effect on the occurrence and development of cervical cancer cells.Pc-HMGB1 reversed the inhibitory effect of matrine on the occurrence and development of cervical cancer cells.Conclusion Matrine inhibits the development and progression of HPV-positive cervical cancer by blocking HMGB1/RAGE signaling pathway.