基于PI3K/AKT/mTOR信号通路探究九味健步饮含药血清对LPS诱导大鼠CP-R092细胞凋亡及炎症的影响OA

Objective:To evaluate the effect of drug-containing serum of Jiuwei Jianbu Decoction on apoptosis and inflammation of LPS-induced rat CP-R092 cells.Methods:The chondrocyte degeneration model was established,the blank control group,model group,Jiuwei Jianbu Decoction drug-containing serum group,LY294002(PI3K inhibitor)group,Jiuwei Jianbu Decoction drug-containing ser-um+LY294002 group were set up.The morphological changes of chondrocytes in each group were observed under the microscope;The cell viability of each group was detected by the CCK-8 method;The apoptosis rate of cells in each group was detected by flow cytometry;Immunofluorescence staining was used to detect the expression of Bax protein;The expressions of PI3K,AKT,mTOR,Bax,Bcl-2,IL-6 and TNF-α mRNA and proteins in chondrocytes were detected by RT-qPCR and Western Blot.Results:Compared with the blank control group,the number of cells in the model group decreased,the morphology changed,the cell viability significantly decreased,the apoptosis rate significantly increased,and the mRNA and protein expressions of PI3K,mTOR,Bax,IL-6,and TNF-α and the AKT mRNA and p-AKT protein significantly increased.The expressions of Bcl-2 mRNA and protein were significantly decreased(P<0.05 or P<0.01).Compared with the model group,the number of cells in each administration group increased,the morphological changes were not signifi-cant,the cell viability increased significantly,the apoptosis rate decreased significantly,the mRNA and protein expressions of PI3K,mTOR,Bax,IL-6,TNF-α and the AKT mRNA and p-AKT protein decreased significantly,and the mRNA and protein expressions of Bcl-2 increased significantly(P<0.05).Conclusion:The drug-containing serum of Jiwei Jianbu Decoction can inhibit the PI3K/AKT/mTOR signaling pathway,alleviate inflammation,and reduce chondrocyte apoptosis.