银杏叶提取物通过调控p38 MAPK/MLCK信号通路对高氧诱导的急性肺损伤小鼠肠黏膜屏障的保护作用OA

Objective To explore the effects of Ginkgo biloba extract(GBE)on the intestinal mucosal barrier in mice with hyperoxia-induced acute lung injury(ALI)via the p38 mitogen-activated protein kinase(MAPK)/myosin light chain kinase(MLCK)signaling pathway.Methods C57BL/6J mice were randomly divided into control,ALI,low-dose GBE,high-dose GBE,and high-dose GBE+anisomycin,with 10 mice in each group.Oxygen partial pressure(PaO2),carbon dioxide partial pressure(PaCO2),and the ratio of lung wet weight to dry weight(W/D)were measured.Histological staining was used to observe and score pathological changes in the lung and intestinal tissues.Serum inflammatory cytokines,D-lactic acid(D-LA),and intestinal sIgA levels were detected using an enzyme-linked immunosorbent assay.The expression of related proteins was assessed by Western blotting.Results Compared with the control group,the ALI group showed significantly decreased PaO2 and expression of occludin,claudin,and zonula occludens-1(ZO-1)proteins(P<0.05),while PaCO2,W/D,lung and intestinal pathological scores,inflammatory cytokines,D-LA,diamine oxidase(DAO),sIgA levels,and p-p38 MAPK/p38 MAPK and MLCK protein expression were significantly increased(P<0.05).Compared with the ALI group,the low-and high-dose GBE groups exhibited opposite changes,with more pronounced improvements in the high-dose GBE group.The high-dose GBE+anisomycin group showed significantly attenuated therapeutic effects compared to the high-dose GBE group(P<0.05).Conclusion GBE may protect the intestinal mucosal barrier by regulating the p38 MAPK/MLCK signaling pathway,reducing inflammation,and increasing the expression levels of intestinal mucosal barrier-related proteins.