云南边境地区稻种资源的SSR标记遗传多样性及其主要农艺性状关联性分析OA

To analyze the genetic diversity of rice germplasm resources in the border areas of Yunnan Province and identify SSR markers closely related to major agronomic traits,a total of 376 newly collected rice germplasm resources from Yunnan(China),and its adjacent Myanmar and Laos were used as experimental materials.These resources were divided into three analysis units,and 11 phenotypic traits were investigated.Genetic diversity and association with phenotypic traits were analyzed using 27 pairs of SSR markers.Results showed that a total of 142 allelic variation loci were detected,including 100 rare allelic variation loci and nine peculiar allelic variation loci.Based on the comprehensive evaluation of six genetic diversity parameters,the scoring order of rice germplasm resources from the three analysis units was:Yunnan,China analysis unit>Myanmar analysis unit>Laos analysis unit.Among the 27 pairs of SSR markers,RM214,RM1086,RM5481,RM6089,RM1509,and RM7479 were closely associated with phenotypic traits,could explain more than 10.0%of the variation in panicle length,panicle exsertion,filled grains per panicle,unfilled grains per panicle,grains per panicle,and 1000-grain weight.Principal component analysis of phenotypic traits extracted four principal components with a cumulative contribution rate of 77.218%.A total of 36 excellent germplasm resources(comprehensive score≥1.000)were selected.Among these,Huake,Nalei 5,and Haonuolang had a score greater than 2.000.They demonstrated potential as parents for multiple-tillering and large-panicle type,large-panicle type,and glutinous type,respectively.