基于KASP技术鉴定新疆春小麦材料抗病基因OA

To identify the distribution of disease resistance functional genes in Xinjiang spring wheat materials and provide materials for genetic improvement of wheat disease resistance breeding,KASP technology was employed to detect genes associated with resistance to stripe rust,leaf rust,powdery mildew,and Fusarium head blight in 549 Xinjiang spring wheat materials.The results showed that 121 materials carried five stripe rust resistance alleles with relatively high frequencies(QYrqin.nwafu-2AL,Yr29,QYrsn.nwafu-1BL,QYrqin.nwafu-6BS,Yr78);352 materials carried two leaf rust resistance alleles with relatively high frequencies(Lr67,Lr46);and 142 materials carried three Fusarium head blight resistance alleles with relatively high frequencies(QFhb.Hbaas-5AS,QFhb.hbaas-5AL,QFhb.caas-5AL).No presence of the five powdery mildew resistance genes(Pm12,Pm21,Pm2a,Pm5e,PmV)was detected in any of the materials.In summary,Xinjiang spring wheat varieties exhibited high frequencies of resistance genes for stripe rust,leaf rust,and Fusarium head blight,but a notable lack of resistance sources for powdery mildew.Therefore,it is crucial to focus on introducing and utilizing new powdery mildew resistant materials to improve the disease resistance breeding capacity of Xinjiang spring wheat.