旺苍茶树资源遗传多样性分析与基因资源挖掘OA
Genetic Diversity Analysis and Gene Resource Exploration of Tea Germplasm Resources in Wangcang
为探明四川省旺苍县茶树种质资源的遗传多样性,挖掘潜在的优异种质用于新品种选育,利用自研的茶树液相功能芯片对136份样品的76252个SNP位点进行了基因型检测,结合理化成分鉴定和感官审评数据,全面分析了旺苍地方资源的遗传结构和品质特征;并通过全基因组关联分析进一步挖掘了品质性状显著关联的SNP位点和候选基因.结果表明,旺苍县域内茶树资源多样性丰富且具有一定的地域性特点,其中大两镇古茶树资源为过渡型茶树群落,富含酯型儿茶素,尤其是表没食子儿茶素没食子酸酯,所制烘青绿茶品质优良,香气普遍高爽/高鲜、有花(果)香,滋味以甘醇/甘和、鲜爽为主.根据芯片检测的40326个核心SNP位点,发掘出4个与茶树氨基酸、茶多酚和表没食子儿茶素没食子酸酯含量高度相关的候选基因,分别是γ-CA1、TPR5、MYB94和UGT85A1.相关研究结果可为今后旺苍茶树种质资源的开发利用提供理论依据.
To clarify the genetic diversity of tea plant germplasm resources in Wangcang county,Sichuan province,and identify potential superior germplasms for new cultivar breeding,this study utilized a self-developed liquid-phase functional chip for tea plant to genotype 76252 SNP loci in 136 accessions.Combined with biochemical component detection and organoleptic evaluation,the genetic structure and quality characteristics of these local germplasms were comprehensively analyzed.Genome-wide association studies were further conducted to identify SNP loci and candidate genes significantly associated with quality traits.The results show that the tea plant germplasm resources in Wangcang county exhibit rich diversity and distinct regional characteristics.Among them,the ancient tea plant population from Daliang town is identified as a transitional type,rich in esterified catechins—especially epigallocatechin gallate.And the baked green tea produced from these plants demonstrates excellent quality,with a generally refreshing and high-aromatic profile featuring floral(or fruity)notes,and a taste characterized by sweetness,mellowness,and freshness.Based on 40326 core SNP loci detected,four candidate genes(γ-CA1,TPR5,MYB94,and UGT85A1)significantly correlated with the content of amino acids,tea polyphenols,and epigallocatechin gallate were identified.These results provide a theoretical foundation for the future development and utilization of tea plant germplasm resources in Wangcang.
王波;郑梦霞;贾丙娟;向云攀;梅飘;陈九江;任勇;马春雷;郝心愿;石保旭
四川省旺苍县茶产业技术研究所,广元 628200中国农业科学院茶叶研究所/农业农村部特种经济动植物生物学与遗传育种重点实验室,杭州 310008中国农业科学院茶叶研究所/农业农村部特种经济动植物生物学与遗传育种重点实验室,杭州 310008四川省旺苍县茶产业技术研究所,广元 628200中国农业科学院茶叶研究所/农业农村部特种经济动植物生物学与遗传育种重点实验室,杭州 310008四川省旺苍县茶产业技术研究所,广元 628200四川省旺苍县茶产业技术研究所,广元 628200中国农业科学院茶叶研究所/农业农村部特种经济动植物生物学与遗传育种重点实验室,杭州 310008中国农业科学院茶叶研究所/农业农村部特种经济动植物生物学与遗传育种重点实验室,杭州 310008四川省旺苍县茶产业技术研究所,广元 628200
旺苍40K茶树液相芯片理化成分感官审评全基因组关联分析
Wangcang40K tea liquid-phase chipbiochemical componentorganoleptic evaluationgenome-wide association study
《植物遗传资源学报》 2026 (2)
387-397,11
四川省区域创新合作项目(2025YFHZ0331)国家农业产业技术体系(CARS-19-02B)Sichuan Provincial Regional Innovation Cooperation Project(2025YFHZ0331)China Agriculture Research System of MOF and MARA(CARS-19-02B)
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