ELISA法与UPLC-MS/MS法检测水产品中喹诺酮类药物残留的比较分析OA

Enzyme-linked immunoassay(ELISA)and ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)were used to detect the effects of 14 quinolones(QNs)in six commercially available aquatic products,aiming to compare the differences and correlations between the two methods.Among them,the detection conditions of the UPLC-MS/MS method were detected and analyzed with reference to the national standard GB/T 20366-2006,and the standard curves of 14 QNs drugs were established,and the quantitative analysis results of the external standard method were compared with the ELISA method.The results showed that the positive detection rate of QNs drug samples was 78.57％by ELISA,64.29％by UPLC-MS/MS,the highest residue of ciprofloxacin was 15.76 μg/kg in the head of yellow catfish,and the positive detection rate of enrofloxacin was 64.29％,and the highest residue was as high as 2128.41 μg/kg.In view of the advantages of ELISA method such as fast analysis speed and simple sample preparation method,it was suitable for the initial screening of large batches of samples,and the UPLC-MS/MS method had high accuracy and good reproducibility,which could be used for the verification of positive results of ELISA method and more accurate determination of drug residue content.This experiment provided a new detection idea for the monitoring of QNs drug residues in aquatic products,and could choose faster and better methods based on the actual working conditions,so as to provide technical support for the detection of QNs drug residues in aquatic products or aquaculture feed additives.