盐酸羟考酮超前镇痛用于结直肠癌大鼠术后效果评价OA
Evaluation of Preemptive Analgesia with Oxycodone Hydrochloride on Postoperative Effects in Rats with Colorectal Cancer
目的 探讨盐酸羟考酮超前镇痛用于结直肠癌大鼠术后的效果.方法 取 72 只SD大鼠,经颈背部皮下注射 1,2-二甲肼(DMH)30 mg/kg、每周 1 次、连续 18 周,以复制结直肠癌大鼠模型,建模成功后持续喂养 16 周,随机分为模型组(0.2mg/kg生理盐水,术前 30 min)、超前镇痛组(2mg/kg,术前 30 min)和术后镇痛组(2mg/kg,术后 30 min),腹腔注射盐酸羟考酮或等体积生理盐水.选择 18 只SD大鼠作为对照组(0.2mg/kg生理盐水,术前 30 min).分别于开腹术前 1 天(D0)和术后第 1 天(D1)、术后第 2 天(D2)对大鼠进行机械刺激和热辐射,检测大鼠的机械痛阈值(PWT)、热痛阈值(PWL);采用酶联免疫吸附试验(ELISA)法检测大鼠血清中一氧化氮(NO)、P物质、皮质醇(Cor)、肾上腺素(E)、去甲肾上腺素(NE)、白细胞介素(IL)17 和IL-22 水平;采用流式细胞术检测外周血辅助性T细胞(Th)17 和Th22 细胞比例.结果 与模型组比较,D1、D2 时超前镇痛组、术后镇痛组的PWT、PWL均显著升高(P<0.05),NO、P物质、Cor、E、NE水平均显著降低(P<0.05);超前镇痛组的IL-17、IL-22、Th17 细胞比例和Th22 细胞比例均显著降低(P<0.05);且超前镇痛组各指标变化幅度均明显大于术后镇痛组(P<0.05).结论 盐酸羟考酮超前镇痛能降低结直肠癌大鼠术后痛感,抑制全身应激反应,并通过调节Th17 和Th22 细胞比例改善细胞免疫功能.
Objective To investigate the effect of preemptive analgesia with oxycodone hydrochloride on postoperative effects in rats with colorectal cancer.Methods A total of 72 SD rats were subcutaneous injected with 1,2-dimethylhydrazine(DMH)30 mg/kg in the cervico-dorsal region,once a week for 18 consecutive weeks to replicate rats with colorectal cancer models.After successful modeling,the rats were continuously fed for 16 weeks and then randomly divided into the model group(0.2 mg/kg normal saline,30 min before operation),the preemptive analgesia group(2 mg/kg,30 min before operation)and the postoperative analgesia group(2 mg/kg,30 min after operation),with oxycodone hydrochloride or equal volume of normal saline were injected intraperitoneally.Another 18 SD rats were selected as the control group(0.2 mg/kg normal saline,30 min before operation).Mechanical stimulation and thermal radiation were used to detect the paw withdrawal mechanical threshold(PWT)and paw withdrawal thermal latency(PWL)of rats on the day before operation(D0),the first day after operation(D1),and the second day after operation(D2).Enzyme-linked immunosorbent assay(ELISA)method was used to detect the serum levels of nitric oxide(NO),substance P,cortisol(Cor),epinephrine(E)and norepinephrine(NE),interleukin(IL)-17 and IL-22 in peripheral blood in rats.Flow cytometry method was used to detect the proportion of helper T cells(Th)17 and Th22.Results Compared with the model group,at D1 and D2,PWT and PWL in the preemptive analgesia group and the postoperative analgesia group were significantly increased(P<0.05),the level of NO,substance P,Cor,E,NE,IL-17,IL-22,the proportion of Th17 cells and Th22 cells in the preemptive analgesia group were significantly decreased(P<0.05),and the change amplitude of all the above indicators in the preemptive analgesia group were significantly greater than those in the postoperative analgesia group(P<0.05).Conclusion Preemptive analgesia with oxycodone hydrochloride can reduce postoperative pain,inhibit systemic stress response,and improve cellular immune function by regulating the proportion of Th17 and Th22 cells in rats with colorectal cancer.
刘卓;张树波;贺子蕗;刘守友;杨立群
华北理工大学附属医院,河北 唐山 063000唐山职业技术学院附属医院,河北 唐山 063000华北理工大学附属医院,河北 唐山 063000河北省唐山市南堡开发区医院,河北 唐山 063000河北省唐山市丰南区医院,河北 唐山 063300
医药卫生
盐酸羟考酮超前镇痛结直肠癌免疫功能
oxycodone hydrochloridepreemptive analgesiacolorectal cancerimmune function
《中国药业》 2026 (4)
86-91,6
河北省医学科学研究课题研究项目[20211806].
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