UPLC-Q-TOF-MS/MS法鉴定3种酚类在小鼠体内的代谢产物OA

AIM To establish a UPLC-Q-TOF-MS/MS method for the identification of in vivo metabolites of gallic acid,ellagic acid and corilagin in mice.METHODS The mice in administration groups were given intragastric administration of 0.5%CMC-Na suspensions of 3 constituents(400 mg/kg),respectively,while those in blank group were given intragastric administration of 0.5%CMC-Na suspensions(400 mg/kg),after which plasma,urine and feces were collected.The analysis was performed on a 30℃thermostatic Acquity UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm),with the mobile phase comprising of water(containing 0.1%formic acid)-acetonitrile flowing at 0.27 mL/min in a gradient elution manner,and electrospray ionization source was adopted in negative ion scanning.RESULTS Total 27 gallic acid metabolites,13 ellagic acid metabolites and 37 corilagin metabolites were identified,these 3 constituents mainly occured phase Ⅰmetabolites such as hydrolysis,decarboxylation,and phase Ⅱ metabolites such as methylation,sulfation,glucuronidation.CONCLUSION This stable and reliabe method can provide a reference for exploring the in vivo metabolic rules of other natural phenols.