翁源巴戟天总环烯醚萜的提取及抗氧化研究OA
Extraction process and antioxidant activity of iridoids from Morinda officinalis
为提高韶关翁源巴戟天总环烯醚萜得率,本试验采用纤维素酶辅助超声法提取,通过单因素试验与Box-Behnken响应面法设计试验优化工艺,并对总环烯醚萜的体外抗氧化活性进行了研究.结果表明:酶辅助超声提取巴戟天总环烯醚萜的最佳工艺条件为:酶量2％、酶解时间2 h、液料比33∶1(mL/g)、超声时间46 min、乙醇浓度39％.此参数下,总环烯醚萜得率为30.86％,与预测值的相对误差为0.16％.此外,巴戟天总环烯醚萜提取物具有一定的抗氧化活性,对超氧阴离子自由基清除的IC50为5.582 mg/mL,同时对钼酸铵有良好的还原能力.
To improve the total iridoids of Morinda officinalis from Shaoguan Wengyuan,the single test and Box-Behnken response surface method were designed by cellulase-assisted ultrasonic extraction,and the antioxidant activity in vitro was investigated.The results showed that the optimal extraction process conditions of total iridoids from Morinda officinalis with enzyme-assisted ultrasonic were:enzyme amount of 2％,enzyme digestion time of 2 h,liquid-to-feed ratio of 33∶1(mL/g),ultrasonic time of 46 min,and ethanol concentration of 39％.Under the condition,the yield of total iridoids was 30.86％,and the relative error to the predicted value was 0.16％.In addition,the total iridoids of Morinda officinalis showed certain antioxidant activity,with an IC50 of 5.582 mg/mL for scavenging superoxide anion radicals and a good reducing ability for ammonium molybdate.
张天华;黄紫红;张旭杭;冯梓健;胡心蕊
广东省粤北食药资源利用与保护重点实验室/韶关学院,广东韶关 512005||韶关学院生物与农业学院,广东韶关 512005韶关学院生物与农业学院,广东韶关 512005韶关学院生物与农业学院,广东韶关 512005韶关学院生物与农业学院,广东韶关 512005韶关学院生物与农业学院,广东韶关 512005
农业科技
巴戟天总环烯醚萜提取工艺响应面法抗氧化活性
iridoids of Morinda officinalisextraction processresponse surface methodologyantioxidant activity
《中国饲料》 2026 (3)
58-63,6
国家自然科学基金青年项目(82103980)韶关市科技计划项目(210728104530489)韶关学院重点科研项目(SZ2020KJ14)
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