酶提及碱提法制备罗汉果多糖的工艺及体外降血糖与抗氧化活性研究OA

The purpose of this study was to extracted polysaccharides from dried Siraitia grosvenorii using both enzymatic and alkaline extractions,and compared their polysaccharide profiles.Linvestigated the effects of enzyme activity,temperature,time,and pH in enzymatic extraction and alkaline concentration,temperature,time,and number of extractions in alkaline extraction,using the rate of polysaccharides of Siraitia grosvenorii as the evaluation criterion through the one-way test and orthogonal test,and optimized the preparation conditions.Meanwhile,their inhibitory effects on α-glucosidase,α-amylase and antioxidant effects were also investigated.The experimental results showed that in the enzyme extraction method,the optimal extraction conditions were enzyme activity of 3000 U,temperature of 60 ℃,time of 150 min,and pH of 5.In the alkaline extraction method,the optimal extraction conditions were alkali concentration of 1 mol/L,temperature of 40 ℃,time of 5 h,and the number of extraction times of 2.The alkaline extraction method yielded the highest extraction rate,which averaged 5.99％and was significantly higher than that of the enzyme extraction method(2.18％).The polysaccharides obtained by the two extraction methods did not differ much in total sugar and glucuronic acid content,and the solubility of the polysaccharides extracted by enzyme was the lowest.The alkali-extracted polysaccharides showed stronger activity in inhibiting α-glucosidase and α-amylase,which suggested their potential application in hypoglycemia.The Siraitia grosvenorii polysaccharides extracted in different ways showed better ability in scavenging DPPH radicals,hydroxyl radicals and superoxide anion,with obvious reducing ability,which indicated that Siraitia grosvenorii polysaccharides had better antioxidant activity.