FKBP5在癫痫大鼠海马的表达及其与突触可塑性损伤的关联OA

Aim To observe the dynamic expression of FK-506 binding protein 5(FKBP5)in the hippo-campal tissue of epileptic rats and its correlation with synaptic plasticity damage.Methods Twenty-four male SD rats were randomly divided into a control group and a model group,with 12 rats in each group.The epileptic model was induced by intraperitoneal in-jection of lithium chloride-pilocarpine,and the seizure latency was recorded.The level of seizures was as-sessed according the Racine grading standard,and cortical electroencephalograms were recorded.The levels of FKBP5 mRNA and protein in hippocampal tis-sues were detected by qRT-PCR and Western blotting.Golgi staining was used to observe the damage of neu-ronal dendritic spines in the hippocampus.Western blot was used to detect the protein levels of PSD95,BDNF,synapsin-1,and synaptotagmin-1.Results SD rats showed typical epileptic seizure behaviors after intraperitoneal injection of lithium chloride-pilocarpine,accompanied by high-amplitude rhythmic spike and sharp wave patterns.Compared to the con-trol group,the hippocampal FKBP5 protein levels in the model group significantly increased at 1,4,and 24 h post-seizure(P<0.05),with the highest protein expression at 24 h(P<0.01).At 24 h post-seizure,the dendritic spine density,branch length and synap-tic plasticity-related protein expression in the hippo-campal neurons of the model group rats were signifi-cantly reduced(P<0.05).Pearson correlation analy-sis revealed that FKBP5 protein expression was signifi-cantly negatively correlated with the expression of PSD95 and synaptotagmin-1 proteins(P<0.05).Con-clusions Intraperitoneal injection of lithium chloride-pilocarpine can successfully induce epileptic models in rats,and the changed expression of FKBP5 in the hippocampus is involved in the mechanism underlying synaptic plasticity impairment in the hippocampus of epileptic rats.