基于指纹图谱与主成分分析评价不同采收期红薯叶的质量OA
Quality evaluation of Ipomoer batatas Poir leaves in different collecting seasons based on HPLC fingerprint and principal component analysis
建立不同采收期红薯叶的高效液相色谱指纹图谱,并对其开展主成分分析,为不同采收期红薯叶的质量控制及品质评价提供参考.以 10 批不同采收期红薯叶为研究样本,探索不同采收期红薯叶HPLC指纹图谱分离条件并对样本进行分析检测,建立不同采收期红薯叶指纹图谱并进行相似度评价,对不同采收期红薯叶进行主成分分析.结果表明,建立的不同采收期红薯叶HPLC指纹图谱的方法稳定、可靠,不同采收期红薯叶指纹图谱中有 10 个共有峰,经对照品指认,确认峰 3 为绿原酸吸收峰.6-7 月采收的红薯叶质量波动较大,与 8 月后样本相比,质量存在较大差异,8 月及后期采收的红薯叶样本质量趋于稳定.10 批不同采收期红薯叶的相似度为 0.720～0.988.不同采收期红薯叶主成分分析共得到 2 个主成分,累计方差贡献率达到 86.849%.本研究结果为不同采收期红薯叶质量控制及其开发的相关农产品的品质评价奠定了基础.
The high performance liquid chromatography fingerprint of Ipomoer batatas Poir leaves in different collecting seasons was established,and the principal component analysis was carried out to provide reference for the quality control and quality evaluation of Ipomoer batatas Poir leaves in different collecting seasons.Taking 10 batches of Ipomoer bata-tas Poir leaves in different collecting seasons as research samples,the separation conditions of HPLC fingerprints of Ipo-moer batatas Poir leaves in different collecting seasons were explored and the samples were analyzed and detected.The fingerprints of Ipomoer batatas Poir leaves in different collecting seasons were established and the similarity was evaluat-ed,and the principal component analysis of Ipomoer batatas Poir leaves in different collecting seasons was carried out.The results showed that the established HPLC fingerprint method of Ipomoer batatas Poir leaves in different collecting seasons was stable and reliable.There were 10 common peaks in the fingerprint of Ipomoer batatas Poir leaves in differ-ent collecting seasons.After identification with reference substances,peak 3 was confirmed as chlorogenic acid absorp-tion peak.The quality of Ipomoer batatas Poir leaves in different collecting seasons from June to July fluctuated greatly,and there was a big difference in quality compared with the samples collected after August.The quality of Ipomoer bata-tas Poir leaves in different collecting seasons collected in August and later period tended to be stable.The similarity of 10 batches of Ipomoer batatas Poir leaves in different collecting seasons was 0.720-0.988.Two principal components were obtained by principal component analysis of Ipomoer batatas Poir leaves in different collecting seasons.And the cumula-tive contribution rate of the two principal components reached 86.849%.The results of this study laid a foundation for the quality control of Ipomoer batatas Poir leaves in different collecting seasons and the quality evaluation of related agricul-tural products developed.
韩忠耀;张智程;余爽爽;刘帮琴;李燕;李江;张林甦
黔南民族医学高等专科学校 贵州都匀 558000黔南民族医学高等专科学校 贵州都匀 558000黔南民族医学高等专科学校 贵州都匀 558000黔南民族医学高等专科学校 贵州都匀 558000黔南民族医学高等专科学校 贵州都匀 558000黔南民族医学高等专科学校 贵州都匀 558000||贵阳康养职业大学 贵阳 550081黔南民族医学高等专科学校 贵州都匀 558000
农业科技
红薯叶高效液相色谱法指纹图谱相似度主成分分析绿原酸
Ipomoer batatas Poir leavesHPLCFingerprintSimilarityPrincipal component analysisChlorogenic acid
《中国瓜菜》 2026 (2)
182-189,8
贵州省自然科学基金(黔科合基础[2020]1Y390 号)贵州省中医药管理局中医药、民族医药科学技术研究基金(QZYY-2025-085,QZYY-2026-090)黔南州科技计划项目(黔南科合社字[2024]5 号)黔南民族医学高等专科学校科研创新团队培育项目(No.Qnyz2024002)黔南民族医学高等专科学校科研基金项目(Qnyz2023004)
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