扭肚藤多糖提取工艺优化及体外抗炎活性评价OA

To optimize the ultrasonic extraction process of polysaccharides from Jasminum elongatum(Bergius)Willd.and study its anti-inflammatory activity in vitro.The total polysaccharide yield from Jasminum elongatum served as the primary response indicator.Ultrasound power,time,extraction temperature,and liquid-solid ratio were selected as independent variables to optimize the extraction process using both single-factor experiments and response surface methodology(RSM).Additionally,the impact on inflammatory factors released by LPS-stimulated RAW264.7 cells was evaluated.The optimal ultrasonic extraction parameters for Jasminum elongatum polysaccharides was obtained as follows:ultrasonic time of 33 min,ultrasonic power of 341 W,extraction temperature of 62 ℃,liquid-solid ratio of 39 mL/g.Under these conditions,the polysaccharide yield reached(6.98±0.34)％.The polysaccharides from Jasminum elongatum exhibited a dose-dependent inhibited the LPS-induced production of TNF-a,IL-1β,and IL-6 while enhancing the generation of IL-10.The optimized ultrasonic extraction process of polysaccharides from Jasminum elongatum is stable and feasible.The polysaccharides from Jasminum elongatum demonstrated anti-inflammatory activity in vitro,which provides theoretical basis for the further research on Jasminum elongatum.