养血补肾方调节CAV1/AQP1通路对形觉剥夺性近视大鼠TGF-β2表达、巩膜重塑及水转运的影响OA

Objective To explore the effects of the Yangxue Bushen formula on transforming growth factor-β2(TGF-β2)expression,scleral remodeling,and water transport in rats with form-deprivation myopia(FDM)by regulating the caveolin-1(CAV1)/aquaporin 1(AQP1)pathway.Methods Forty SPF-grade male SD rats were collected.Thirty rats were selected to establish FDM models.After successful modeling,they were divided into the FDM group,the pirenzepine(PIR)group,and the Yangxue Bushen formula group,with 10 rats in each group.Additionally,10 rats that were not modeled were classified as the normal group.Rats in the normal group and the FDM group received no intervention.Rats in the PIR group were given PIR eye drops in the morning and evening every day for intervention,while rats in the Yangxue Bushen formula group were given the Yangxue Bushen formula by gavage for intervention.The refractive power and axial length of rats were detected by retinoscopy and diagnostic instruments.The morphological changes in the scleral tissue of rats were detected by hematoxylin-eosin(HE)staining.The TGF-β2 expression in the scleral tissue of rats was detected by immuno-histochemical staining.The relative expression levels of AQP1 and CAV1 in the scleral tissue of rats were measured by West-ern blot,and the relative mRNA expression levels of aspartate transporter(GLAST)and glutamate transporter 1(GLT-1)in the scleral tissue of rats were measured by real-time polymerase chain reaction.Results Compared with the normal group,the refractive power,the expression level of TGF-β2,and the relative expression levels of AQP1 and CAV1 proteins in the FDM group,the Yangxue Bushen formula group,and the PIR group decreased,while the axial length and the relative ex-pression levels of GLAST and GLT-1 mRNAs increased(all P＜0.05).Compared with the FDM group,the refractive power,the expression level of TGF-β2,and the relative expression levels of AQP1 and CAV1 proteins in the Yangxue Bushen formu-la group and the PIR group increased,while the axial length and the relative expression levels of GLAST and GLT-1 mRNAs decreased(all P＜0.05).Compared with the Yangxue Bushen formula group,the refractive power,the expression level of TGF-β2,and the relative expression levels of AQP1 and CAV1 proteins in the PIR group decreased,while the axial length and the relative expression levels of GLAST and GLT-1 mRNAs increased(all P＜0.05).The results of HE staining showed that there were no abnormal changes in the structure of each layer of the retina in the normal group.In the FDM group,the reti-na of rats became thinner,and severe vacuolar changes occurred in the ganglion cell layer.The retinal thickness of rats in the Yangxue Bushen formula group and the PIR group increased,the cell arrangement was basically regular,the number of ganglion cells increased,and the cells in each layer were densely arranged.Among them,the rats in the Yangxue Bushen for-mula group showed the most significant performance.Conclusion The Yangxue Bushen formula has a positive effect on FDM,which may be related to the regulation of CAV1,AQP1,and TGF-β2 expression levels,and balance and improvement of scleral remodeling and water transport functions.