Quick TargSeqTM全集成DNA快速检测系统在极端环境下运行性能评测OA
Performance Verification of Quick TargSeqTM Rapid DNA Integrated System under an Extreme Environment
快速DNA分析技术能够将DNA提取、多重PCR扩增以及毛细管电泳等多个步骤集成,无需人工干预即可自动完成所有流程.商业化的DNA快速检测系统可通过自动化设备提供的内部环境来模拟实验室环境,从而使得整个检测过程彻底摆脱常规DNA实验室所需环境条件限制.本文针对国产Quick TargSeqTM全集成DNA快速检测系统,开展极端环境条件下作业能力测试,评估其实战使用效能,这是在该领域的首次尝试.将Quick TargSeqTM装载于车辆上,进行车载静止测试和行进中测试.测试高度涵盖从海拔500 m的平原环境到海拔3 800 m的高原环境,共分为4个静止测试点(包含1个平原参考点)和5个行进中测试路段.测试全程为雨雪天气,最低气温达-1℃,总计测试数据36组,其中平原静止点2组,平原行进中测试4组,高原静止点18组,高原行进中测试12组.测试结果显示STR基因座的检出率为99.21%,分型准确率为100%,测试结果表明该快检系统具备环境适应能力和野外作业能力.
Rapid DNA analysis technology represents an innovative approach by integrating multiple stages of the DNA analysis process,including DNA extraction,multiplex PCR amplification,and capillary electrophoresis.This analysis technology enables the automatic execution of all these processes,eliminating the need for manual intervention.Commercially available rapid DNA testing systems can simulate the laboratory environment through the internal environment offered by automated equipment.This advancement liberates the entire testing procedure from the stringent environmental conditions typically required in a DNA level three laboratory.In this article,the performance of the domestic fast integrated forensic DNA analyzer Quick TargSeqTM was verified under extreme environments.The tested instrument was loaded onto an SUV for both on-vehicle and on-the-move testing.The tests ranged from an altitude of 500 m on the plain to a peak altitude of 3800 m,which included 4 park-and-test points(with 1 reference point on the plain)and 5 on-the-move test trips.Throughout the test,there was a mix of rainy and snowy weather,with environmental temperatures dropping as low as-1℃.A total of 36 results were collected,which included 2 from reference points at plain,4 from on-the-move trip at plain,18 from park-and-test points at highland and 12 from on-the-move trip at highland.Taking all the collected data into calculation,the loci detection rate of the test is 99.21%and the loci accuracy is 100%.The test results indicate that the Quick TargSeqTM DNA system is highly environmental adaptable and capable of operating effectively in both highland and on-vehicle settings.
黄章龙;韩俊萍;庄斌;谢何鑫;李彩霞;张海军
四川省公安厅物证鉴定中心,成都 610041北京市公安局朝阳分局刑侦支队,北京 100025北京博奥晶典生物技术有限公司,北京 101111北京博奥晶典生物技术有限公司,北京 101111公安部鉴定中心,法医遗传学公安部重点实验室,北京市现场物证检验工程技术研究中心,现场物证溯源技术国家工程实验室,北京 100038四川省公安厅物证鉴定中心,成都 610041
社会科学
法医物证学DNA检验快速DNA高原测试低温雨雪环境
forensic geneticsDNA analysisrapid DNAplateau testlow-temperature rainy and snowy weather
《刑事技术》 2026 (1)
16-21,6
北京市现场物证检验工程技术研究中心开放课题(2021CSEEKFKT06)
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