基于NMDA通路探讨茸菖胶囊干预月经性癫痫神经元模型的抗痫机制OA

[Objective]To observe the effect of Rongchang Capsules on N-Methyl-D-aspartic acid(NMDA)receptor in the neuronal model of catamenial epilepsy,and to explore its mechanism.[Methods]The magnesium-free epileptic neurons induced by exogenous E2(catamenial epileptic neuron model)were randomly divided into 7 groups:control group,epilepsy model group,catamenial epilepsy model group,sodium valproate group and Rongchang Capsules low dose group,Rongchang Capsules middle dose group and Rongchang Capsules high dose group.The neuronal morphology,discharge frequency,resting membrane potential,24 h NMDA current changes,NMDA Receptor 1(NR1)and NMDA Receptor 2A(NR2A)receptor protein expression were observed.[Results]Compared with the control group,the high-frequency and high-amplitude spike discharges in the catamenial epilepsy model group were significantly increased;the number of neurons was significantly decreased;the discharge frequency was significantly increased;the resting membrane potential was significantly increased;the 24 h NMDA channel current density was significantly increased(P<0.05 or P<0.01);the expression of NR1 protein was significantly decreased,and the expression of NR2A protein was significantly increased(P<0.05 or P<0.01).Compared with the catamenial epilepsy model group,the high-frequency high-amplitude spike wave discharge in the sodium valproate group was significantly decreased;the number of neurons increased;the discharge frequency decreased significantly(P<0.05);the resting membrane potential and the 24 h NMDA channel current density was not decreased significantly(P>0.05);the expression of NR1 and NR2A protein did not change significantly(P>0.05);the high-frequency and high-amplitude spike wave discharge in the Rongchang capsules high dose group was significantly decreased;the number of neurons was significantly increased;the discharge frequency was significantly decreased;the resting membrane potential was significantly decreased and the 24 h NMDA channel current density was significantly decreased(P<0.05),and the expression of NR2A protein was significantly decreased(P<0.05),and the expression of NR1 protein was not significantly increased(P>0.05).[Conclusion]Rongchang Capsules may reduce the abnormal excitability of NMDA receptor-mediated nerves by down-regulating the expression of NR2A protein,thereby reducing the abnormal discharge of magnesium-free epileptic neurons supplemented with exogenous E2,thus exerting anti-epileptic effects.