原发性肝细胞癌患者XRCC1基因rs25487多态性与血清甲胎蛋白水平的相关性分析OA
Correlation between rs25487 polymorphism of XRCC1 gene and serum alpha-fetoprotein level in patients with primary hepatocellular carcinoma
目的 探究原发性肝细胞癌(hepatocellular carcinoma,HCC)患者X线修复交叉互补蛋白 1(X-ray repair cross complementing group 1,XRCC1)基因单核苷酸多态性与血清甲胎蛋白(alpha-fetoprotein,AFP)水平的关系.方法 选取 2014年 1 月至 2024 年 9 月在青岛大学医学院附属烟台毓璜顶医院接受手术治疗的 380 例原发性HCC患者(HCC组)和进行体检的 361 例健康人(对照组).采用聚合酶链反应-限制性片段长度多态性技术(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)检测XRCC1 基因rs25487 位点基因型.采用化学发光仪测定血清AFP水平.采用线性回归分析XRCC1基因的rs25487 位点多态性与血清AFP的相关性.结果 在HCC组rs25487 共显性模型中,GG型(n=167)、GC型(n=101)和CC型(n=112)中血清AFP水平分别为(56.88±72.85)、(110.91±315.90)和(342.12±1 698.08)ng/mL(P＜0.01).与GG型比较,携带GC型和CC型的HCC患者血清AFP水平的平均值分别上升 95.0%(P=0.044)或 501.4%(P＜0.01).在显性模型中,与GG型比较,携带GC/CC型的HCC患者血清AFP水平的平均值上升 308.7%[(232.49±1 253.08)ng/mL vs(56.88±72.85)ng/mL,P＜0.01].在隐性模型中,与GC/GG型比较,携带CC型的HCC患者血清AFP水平的平均值上升342.9%[(342.12±1 698.08)ng/mL vs(77.24±203.38)ng/mL,P＜0.01].在多元线性回归模型下分析显示,rs25487 等位基因型与HCC患者血清AFP水平相关(P＜0.01).结论 XRCC1基因的rs25487 与HCC患者的血清AFP水平具有独立相关性.携带XRCC1基因的rs25487 位点的C等位基因的HCC患者血清AFP水平升高.
Objective To investigate the association between the single nucleotide polymorphism of X-ray repair cross com-plementing group 1(XRCC1)gene and serum alpha-fetoprotein(AFP)level in patients with hepatocellular carcinoma(HCC).Methods A total of 380 patients with primary HCC who underwent surgical resection and 361 healthy individuals who had phys-ical examination at Affiliated Yantai Yuhuangding Hospital,Qingdao University Medical College,between January 2014 and Sep-tember 2024,were recruited as the HCC group and control group,respectively.The genotyping of the XRCC1 rs25487 locus was performed using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Serum AFP level was quan-tified using a chemiluminescence immunoassay analyzer.The relationship between rs25487 polymorphism and serum AFP lev-el was assessed using linear regression analysis.Results In the codominant model of the HCC group,serum AFP levels were(56.88±72.85),(110.91±315.90),and(342.12±1 698.08)ng/mL for the GG(n=167),GC(n=101),and CC(n=112)genotypes(P＜0.01).Compared to those of the GG genotype,the carriers of the GC and CC genotypes exhibited a 95.0%increase(P=0.044)and a 501.4%increase(P＜0.01)in the mean serum AFP level,respectively.In the dominant model,the carriers of the GC/CC geno-type showed a 308.7%increase in the mean serum AFP level compared to that with the GG genotype[(232.49±1 253.08)ng/mL vs(56.88±72.85)ng/mL,P＜0.01].In the recessive model,the carriers of the CC genotype exhibited a 342.9%increase in the mean se-rum AFP level compared to that of the GC/GG genotype[(342.12±1 698.08)ng/mL vs(77.24±203.38)ng/mL,P＜0.01].Multiple lin-ear regression analysis confirmed that rs25487 genotype was independently associated with serum AFP level in HCC patients(P＜0.01).Conclusions The rs25487 polymorphism of XRCC1 gene is independently associated with serum AFP level in HCC patients.The carriers of the C allele at the XRCC1 rs25487 locus have significantly elevated serum AFP level.
殷浩铭;潘正龙;朱俊涛;刘小方
青岛大学医学院附属烟台毓璜顶医院肝胆胰脾外科,山东 烟台 264000青岛大学医学院附属烟台毓璜顶医院肝胆胰脾外科,山东 烟台 264000青岛大学医学院附属烟台毓璜顶医院肝胆胰脾外科,山东 烟台 264000青岛大学医学院附属烟台毓璜顶医院肝胆胰脾外科,山东 烟台 264000
肝细胞癌单核苷酸多态性甲胎蛋白X线修复交叉互补蛋白1rs25487
hepatocellular carcinomasingle nucleotide polymorphismalpha-fetoproteinX-ray repair cross complementing group 1rs25487
《实用肿瘤杂志》 2026 (1)
66-71,6
湖北陈孝平科技发展基金会青年科学专项基金(CXPJJH123001-2319)
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