多发性骨髓瘤组织circ-PTK2和circ-ITCH表达及其与患者临床病理特征和预后的关系OA

Objective To investigate the expressions of circular RNA protein tyrosine kinase 2(circ-PTK2)and circular RNA itchy E3 ubiquitin protein ligase(circ-ITCH)in multiple myeloma(MM)tissues,and their relationship with the clinicopathological characteristics and prognosis of MM patients.Methods A total of 93 MM patients admitted to our hospital from January 2017 to August 2020 were select-ed as the MM group,and 93 normal bone marrow specimen from our hospital collected during the same period were selected as the control group.qPCR was used to detect the expressions of circ-PTK2 and circ-ITCH in tissues.Based on the follow-up results within three years,the patients were divided into the death group(n=42)and survival group(n=51).Kaplan-Meier method was applied to analyze the relation-ship between the expressions of circ-PTK2 and circ-ITCH and the prognosis of MM patients.Multivariate logistic regression was applied to analyze the factors affecting the prognosis of MM patients.Receiver operating characteristic(ROC)curve was used to evaluate the value of circ-PTK2 and circ-ITCH expressions in predicting the poor prognosis of MM patients.Results Compared with the control group,the expression of circ-PTK2 was higher in the MM group,while the expression of circ-ITCH was lower(both P＜0.05).The expressions of circ-PTK2 and circ-ITCH were associated with International Staging System(ISS)staging and serum creatinine(Scr)and albumin(Alb)levels(all P＜0.05).Kaplan-Meier survival curves showed that grouped by the mean expression level,the 3-year overall survival(OS)rate of patients with high circ-PTK2 expression was lower than that of patients with low circ-PTK2 expression,and the 3-year OS rate of patients with low expression of circ-ITCH was lower than that of patients with high expression of circ-ITCH(both P＜0.05).There were significant differ-ences in ISS stage,the levels of serum Alb and Scr,and the expressions of circ-PTK2 and circ-ITCH between the death group and survival group(all P＜0.05).Logistic analysis showed that ISS staging,serum Scr level,and high circ-PTK2 expression were risk factors affecting the prognosis of MM patients,while high circ-ITCH expression was a protective factor(all P＜0.05).The ROC AUC of the combination of circ-PTK2 and circ-ITCH expressions on predicting the prognosis of MM patients was 0.878,which was larger than that of circ-PTK2 or circ-ITCH expression alone(both P＜0.05).Conclusions Circ-PTK2 is highly expressed in MM tissues,while circ-ITCH is lowly expressed,which is related to the clinicopathological characteristics and prognosis of patients.