不孕女性患者红细胞叶酸水平与IVF-ET结局的相关性OA

Objective To explore the effect of red blood cell(RBC)folate levels on the outcomes of in vitro fertilization-embryo transfer(IVF-ET)in infertile women,and evaluate its potential role in follicular recruitment,oocyte maturation,oocyte fertilization,and early embryo development during assisted reproductive cycles.Methods A total of 105 infertile women who underwent IVF-ET treat-ment from January to December 2021 in the Reproductive Center of the Department of Obstetrics and Gynecology,Xijing Hospital,Air Force Medical University were enrolled in this retrospective study.RBC folate level was measured two days before oocyte retrieval.The outcomes were compared in terms of the number of retrieved oocytes,mature oocytes,two pronuclei(2PN)oocytes,2PN cleavage embryos,and transferable embryos.Linear regression analysis was used to examine the correlation between RBC folate levels and IVF-ET outcome indicators.The subjects were further divided into≥800 nmol/L group and<800 nmol/L group according to red blood cell folate levels.Independent sample t-test was used to compare the outcome indicators of IVF-ET between the two groups,and the relationship between RBC folate level and IVF-ET outcomes was further analyzed.Results Linear regression analysis showed no significant correlation between RBC folate level and the numbers of retrieved oocytes,mature oocytes,and transferable embryos(P>0.05),but a marginal negative correlation between RBC folate level and the numbers of 2PN oocytes and 2PN cleavage embryos(P value close to 0.050).Further analysis after grouping revealed that the number of 2PN oocytes,2PN cleavage embryos,and transferable embryos were significantly lower in RBC folate≥800 nmol/L group than in<800 nmol/L group(P<0.05).Conclusion When RBC folate accumulates to a certain threshold in the body,it may have an adverse effect on oocyte fertilization and early embryo development.Therefore,folic acid supplementation in assisted reproductive populations should be individualized based on individual levels and metabolic status.