高纯度超分子弹性蛋白规模化生产及功效OA

This study aims to develop efficient purification techniques for supramolecular elastin and systematically evaluate its biological efficacy in cell repair and skin care,providing a theoretical basis for its application in the skincare field.The purification process was optimized through nickel column affinity chromatography for large-scale production,and the protein self-assembled into hydrogels to enhance stability.With regards to the effects on human skin fibroblasts,various cellular methods were utilized for the assessment,including proliferation assay,scratch migration assay,and cell adhesion assay(concentration gradient of 0.5-10 g/L).In addition,the human skincare efficacy was verified through skin gloss analysis,moisture content detection,and elasticity measurement.Starting from the protein purified under optimized conditions,supramolecular elastin was successfully prepared,giving excellent purity(up to 98.5%).More importantly,the formed hydrogel demonstrated great stability at room temperature,without any visible degradation within 12 months of storage.At cellular level,supramolecular elastin could greatly promote cell proliferation,with 23.91%and 57.94%improvement at concentrations of 0.5 μg/mL and 1 μg/mL,respectively.Cell adhesion could also be improved,with 82.22%enhancement by treatment with 10 g/L hydrogel.Human tests were next conducted for the use of 30 days,and positive results were obtained at the end of the studies.In details,skin gloss increased by 29.2%,moisture content rose by 27.5%,and skin elasticity improved by 11.9%(P＜0.01).In summary,a large-scale purification process was established for reliable production of high-purity supramolecular elastin,which demonstrated significant improvement of skin physiological indicators by promoting fibroblast activity.Experimental data also showed the potential application of this protein in skincare areas such as moisturizing and anti-aging,providing scientific ground for the development of new bioactive skincare products.