响应面法优化菌酶协同发酵配合饲料工艺研究OA

The purpose of the study was to optimize the solid-state fermentation process on compound feed by collaboration of microorganisms and protease.The relative content of acid-soluble protein to crude pro-tein was used as the evaluation index in this research,the effects of protease amount,fermentation tempera-ture,moisture content,inoculated amount and fermentation time on the feed fermentation were discussed through single-factor experiments.The relevant parameters of fermentation process were optimized to deter-mine the optimal conditions of feed fermentation by response surface methodology based on Box-Behnken design.The results showed that moisture content,inoculated amount and fermentation time were the main factors influencing the fermentation effects.After the optimization,the optimal fermentation parameters were as follows:protease addition amount 0.1%,moisture content 41%,inoculation 1.7×109 CFU/kg and fermen-tation time 7.3 d.Under optimal fermentation conditions,the relative content of acid-soluble protein to crude protein of the fermented compound feed was 30.95%,the pH value decreased to 4.51,the prote-ase activity increased to 154.53 U/g,and the deg-radation rates of glycinin and β-conglycinin increased to 62.76%and 65.56%,respectively.