基于网络药理学探讨黄芩苷缓解AFB1诱导肝细胞凋亡的靶点和通路OA
Exploring Targets and Pathways of Baicalin to Alleviate AFB1-Induced Apoptosis in Hepatocytes Based on Network Pharmacology
研究采用网络药理学的方法,旨在探讨黄芩苷缓解AFB1诱导肝细胞凋亡的靶点和通路.首先,通过PharmMapper数据库、SwissTargetPrediction数据库、TCMSP数据库、Metascape数据库归纳黄芩苷、黄曲霉毒素B1(AFB1)和肝细胞凋亡的靶点,构建Venn图.其次,运用Cytoscape 3.7.2软件进行蛋白质相互作用(PPI)交互网络构建.再次,应用Metascape数据库对关键靶点进行基因本体(GO)功能注释和京都基因与基因组百科全书(KEGG)富集分析.最后,通过分子对接技术分析黄芩苷与核心靶点结合能力.基于PharmMapper数据库、SwissTargetPrediction数据库、TCMSP数据库获取黄芩苷靶点共370个,基于GeneCards数据库、OMIM数据库归纳出AFB1致肝损伤靶点共148个,通过Venn分析,筛选得到黄芩苷与AFB1致肝损伤共同交集靶点有17个,利用STRING数据库构建PPI互作网络,并结合Cytoscape软件分析进一步筛选出黄芩苷缓解AFB1致肝损伤的关键靶点,筛选得到黄芩苷靶点81个,AFB1致肝损伤靶点51个,交集靶点14个,分别为JNK1、GPT、TLR4、HPRT1、CYP1A1、HMOX1、BAX、TP53、PTGS2、Caspase3、MAPK1、EGFR、TGFB1、CYP1A2.GO富集分析发现,黄芩苷缓解AFB1致肝细胞凋亡主要涉及氧化应激、线粒体损伤等相关生物进程;KEGG富集分析表明,在黄芩苷缓解AFB1诱导肝细胞凋亡中起主要作用的是丝裂原活化蛋白激酶(MAPK)信号通路.分子对接结果显示,黄芩苷具有靶向JNK1、TGFB1、EGFR、MAPK1、BAX等靶点的潜力.基于网络药理学的结果,研究发现,黄芩苷可能通过靶向JNK1、GPT、TLR4、HPRT1、CYP1A1、HMOX1、BAX、TP53、PTGS2、Caspase3、MAPK1、EGFR、TGFB1、CYP1A2相关靶点,参与MAPK等相关信号通路,缓解了AFB1诱导肝细胞凋亡的作用.
The aim of this study was to investigate the target and pathway of baicalin in alleviating AFB1-induced hepatocyte apoptosis by using network pharmacology.Firstly,the targets of baicalin,AFB1 and hepatocyte apoptosis were summarized by PharmMapper database,SwissTargetPrediction database,TCMSP database and Metascape data-base to build venn diagram.Secondly,PPI interac-tive network is constructed using Cytoscape 3.7.2 software.Then,the Metascape database is used for the key target for gene ontology(GO)function annotation and kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis.Finally,the ability to analyze the combination of baicalin and core targets through molecular docking technology.Based on Pharmmapper,Swiss Target Prediction,TCMSP database obtained 370 targets of baicalin,based on Genecards and OMIM databases,148 targets of liver injury caused by AFB1.Through Venn analysis,there are 17 common inter-section targets to obtain baicalin and AFB1.Use the String database to build a PPI interactive network,and combine the Cytoscape software analysis to further screen the key target of baicalin to relieve AFB1.There are 81 targets for baicalin,51 targets of liver injury caused by AFB1,and 14 intersection targets,namely JNK1,GPT,TLR4,HPRT1,CYP1A1,HMOX1,BAX,TP53,PTGS2,Caspase3,MAPK1,EGFR,TGFB1,CYP1A2.GO enrichment analysis showed that baicalin alleviated AFB1-induced hepatocyte apoptosis mainly involved oxidative stress,mitochondrial damage and other related biological processes;KEGG enrichment analysis showed that Mitogen-Activated Protein Kinase(MAPK)signaling pathway played a major role in baicalin alleviating AFB1 induced hepatocyte apoptosis.Baicalin has the potential to target JNK1,TGFB1,EGFR,MAPK1,BAX and other targets.Based on the results of network pharmacology,this study discussed that baicalin may participate in MAPK and other related signaling pathways by targeting JNK1,GPT,TLR4,HPRT1,CYP1A1,HMOX1,BAX,TP53,PTGS2,Caspase3,MAPK1,EGFR,TGFB1,CYP1A2 related targets,and play a role in alleviating AFB1 induced hepatocyte apoptosis.
朱意;闻德锋;周华林;邱银生;熊江林;吴仲元;陆启荣
武汉轻工大学动物科学与营养工程学院,动物营养与饲料科学湖北省重点实验室,湖北 武汉 430023武汉轻工大学动物科学与营养工程学院,动物营养与饲料科学湖北省重点实验室,湖北 武汉 430023襄阳职业技术学院农学院,湖北 襄阳 441050武汉轻工大学动物科学与营养工程学院,动物营养与饲料科学湖北省重点实验室,湖北 武汉 430023武汉轻工大学动物科学与营养工程学院,动物营养与饲料科学湖北省重点实验室,湖北 武汉 430023武汉轻工大学动物科学与营养工程学院,动物营养与饲料科学湖北省重点实验室,湖北 武汉 430023武汉轻工大学动物科学与营养工程学院,动物营养与饲料科学湖北省重点实验室,湖北 武汉 430023
农业科技
黄芩苷肝细胞凋亡AFB1网络药理学靶点
baicalinhepatocyte apoptosisAFB1network pharmacologytarget
《饲料工业》 2026 (3)
86-92,7
湖北省重点研发计划项目[2023BBB069]动物营养与饲料科学湖北省重点实验室开放课题项目[202313]
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