HPLC-MS/MS快速测定鸡肉中酰胺醇类和硝基咪唑类药物OA

To achieve the rapid and simultaneous determination of amide alcohol and nitroimidazole drug residues in chicken,a high-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)analytical detection technique was established in this paper.The chicken samples were homogenized and crushed,pre-mixed and dispersed with water,extracted with acetonitrile,purified in one step with a Captiva EMR-Lipid solid-phase extraction column,and determined by HPLC-MS/MS.The amide alcohol and nitroimidazole drugs were quantified by the matrix-matched standard solution internal standard method and the matrix-matched standard solution external standard method,respectively.The amide alcohol and nitroimidazole drugs showed good linear relationships within the concentration range of 0.3 to 10.0 µg/kg.The detection limits were both 0.1 µg/kg,and the quantification limits were both 0.3 µg/kg.At the levels of 1.0、2.0 and 5.0 µg/kg,the recovery rates of the amide alcohol drugs ranged from 95.5%to 105.8%,and the recovery rates of the nitroimidazole drugs ranged from 82.4%to 109.3%,with relative standard deviations ranging from 0.4%to 10.1%.This method is simple and rapid,and meets the relevant regulations and requirements for the detection of amide alcohol and nitroimidazole drug residues in chicken.