湖羊GRB10基因组织表达、SNP扫描及其与血液生理指标关联分析OA

Growth factor receptor-bound protein 10(GRB10),as a key gene regulating growth and development as well as energy metabolism,plays a significant role in immune regulation.Blood physiological indicators serve as important reference criteria for assessing animal's health status,In practical production,monitoring blood physiological indicators enables timely understanding of animal's health status and immune capacity,while alterations in immune performance also influence blood physiological indicators.In this study,a total of 1 086 healthy Hu sheep(Ovis aries)with clear pedigrees were selected as research subjects.The correlations among their hematological and physiological indices were analyzed,and the effects of GRB10 gene polymorphisms on these indices were investigated.Results revealed that there were strong positive correlations among similar blood physiological indicators,weaker correlations between platelet traits and red blood cell/white blood cell traits,and significant negative correlations among white blood cell-related traits.The coefficient of variation for blood physiological indicators in the experimental group ranged from 6.50%to 66.67%,indicating high variability.PCR amplification,Sanger sequencing,and AQP genotyping of the GRB10 gene identified the mutation site GRB10 g.6478288 G>A located in the sixth intron,exhibiting moderate polymorphism.The association analysis results indicated that individuals with the GG genotype exhibit significantly higher mean corpuscular hemoglobin concentration(MCHC),total white blood cell count(WBC),lymphocyte count(LYMPH1),eosinophil count(EO1),and reticulocyte hemoglobin equivalent(RET_He)were significantly higher than those of AA genotype individuals(P＜0.05).Conversely,GG genotype individuals exhibited significantly lower hematocrit(HCT),mean corpuscular volume(MCV),and red blood cell hemoglobin(RBC_He)levels compared to AA genotype individuals(P＜0.05).In summary,the GRB10 g.6478288 G>A variant may serve as a molecular marker for sheep-related blood physiological indicators.This study provides theoretical guidance for enhancing sheep immune performance and increasing breeding efficiency,while offering scientific basis for disease prevention and treatment.