基于网络药理学探究胆木对白色念珠菌的抑制作用OA

This study employs network pharmacology,molecular docking,and antibacterial experiments to explore the key action targets and potential mechanisms by which the active components of the Li ethnic medicine N.officinalis inhibit Can-dida albicans.Targets of the active components of N.officinalis were obtained from the CNKI,PubMed,and SwissTarget databases,while C.albicans-related targets were retrieved from the DisGeNET database.The intersection of these targets was input into the STRING database to obtain core targets,which were then subjected to enrichment analysis,molecular docking,and experimental validation.The results showed that nine active components,258 corresponding targets,and 832 disease tar-gets were screened out,with 43 common targets obtained from the intersection,mainly including IL6,ALB,STAT3,PPARG,EGFR,etc.Enrichment analysis indicated that the pathways through which N.officinalis inhibits C.albicans infection mainly involve the regulation of inflammatory responses by antigen stimulation,the positive regulation of cytokine production in inflammatory responses,and inflammatory response pathways.Molecular docking revealed that the active components of N.officinalis can bind spontaneously to core proteins.Antibacterial experiment results showed that the N.officinalis extract reached MIC at 250 μg·mL-1.This study explored the active components and mechanisms of N.officinalis in treating C.albicans infection via network pharmacology,molecular docking,and antibacterial experiments and found that N.officinalis exerts therapeutic effects through multitarget,multicomponent,and multipathway actions.