代谢组和转录组联合分析网棚种植对脆蜜金柑果皮代谢物的影响OA

To compare the effects of open-field cultivation and net-house cultivation on metabolites and gene transcription levels in the peel of Cuimi Kumquat,screen out key genes involved in the expression of flavonoid synthase,and analyze the regulatory modes of related genes,to provide a reference for improving the flavonoid content in the peel.Taking 8-year-old Cuimi Kumquat as the experimental material,open-field cultivation was used as the control group,and net-house cultivation was used as the experimental group.The differences in the transcriptome and metabolome of the Cuimi Kumquat peel under different cultivation methods were compared.The results showed that compared with open-field cultivation,net-house culti-vation resulted in 84 upregulated metabolites,178 downregulated metabolites,and 2 448 metabolites with no significant dif-ference in Cuimi Kumquat peel,among which differential flavonoids accounted for 30.3%of the total differentially abundant metabolites.A total of 1088 genes were significantly upregulated,and 843 genes were significantly downregulated.Fifty-two differentially abundant metabolites could be annotated by the KEGG database and were enriched in 35 pathways,including flavonoid biosynthesis.Nine hundred and thirteen differential genes could be annotated by the KEGG database and enriched in 136 pathways,including the photosynthesis-antenna protein pathway.Pathways closely related to flavonoid synthesis included the phenylpropanoid biosynthesis pathway(ko00940),flavonoid biosynthesis pathway(ko00941),and flavone and flavonoid biosynthesis pathway(ko00944).Key genes potentially regulating flavonoid synthesis,including PAL,C4H,4CL,and CHI,were screened out.Compared with open-field cultivation,net-house cultivation led to the downregulation of various metabo-lites in Cuimi Kumquat peel,and the potential key genes regulating flavonoid synthesis were PAL,C4H,4CL,and CHI.