荧光定量PCR法与液体培养法检测解脲脲原体结果比较及阳性标本的药敏分析OA
Comparison of Ureaplasma urealyticum detection results by fluorescence quantitative PCR and liquid culture methods,and drug susceptibility analysis of positive specimens
目的 比较荧光定量PCR法与液体培养法检测解脲脲原体(Uu)的阳性率,并对单纯Uu阳性标本进行药敏分析,为临床上Uu感染的诊断和治疗提供依据.方法 回顾性分析重庆市中医院2022 年 1 月—2024 年 12 月368 例采用荧光定量PCR法和液体培养法进行Uu检测患者的结果,比较两种方法检测的阳性率,分析两种方法检测均阳性的样本资料,并对单纯Uu阳性标本进行药敏分析.结果 荧光定量PCR法与液体培养法对Uu检测的阳性率差异有统计学意义(P=0.004),两种方法检测Uu均为阳性的患者71 例,阳性率为 19.29%,其中女32 例(35.56%),男39 例(14.03%),女性比例明显高于男性(x2=20.23,P＜0.001).仅液体培养法阳性的98 例,阳性率为26.63%;仅PCR法阳性的80 例,阳性率为21.74%.两种方法检测结果一致性为中等(Kappa=0.734).两种方法检测Uu均阳性的患者年龄主要集中在 20～39 岁.单纯Uu阳性 58 例,对美满霉素和交沙霉素的敏感率均为100%,对强力霉素和克拉霉素的敏感率均在 90%以上.结论 荧光定量PCR法和液体培养法对Uu检测的阳性率有差异,必要时考虑两种方法联合检测以提高检测的准确性.临床上对Uu阳性患者进行治疗时可将敏感性较高的美满霉素、交沙霉素、强力霉素和克拉霉素作为首选.
Objective To compare the positive rates of fluorescence quantitative PCR and liq-uid culture methods in detecting Ureaplasma urealyticum(Uu)and to conduct drug susceptibility analysis on specimens with isolated Uu positivity,thereby providing evidence for the clinical diag-nosis and treatment of Uu infections.Methods A retrospective analysis was performed on the re-sults of Uu detection by PCR and liquid culture methods in 368 cases at Chongqing Traditional Chinese Medicine Hospital from January 2022 to December 2024.The positive rates of Uu detec-tion by the two methods were compared.The data from samples positive for Uu by both methods were analyzed,and drug susceptibility analysis was conducted on specimens with isolated Uu posi-tivity.Results There was a significant difference in the positive rates of Uu detection between PCR and liquid culture methods(P=0.004).A total of 71 patients were positive for Uu by both methods,yielding a positive rate of 19.29%,including 32 females(35.56%)and 39 males(14.03%).The proportion of females was significantly higher than that of males(x2=20.23,P＜0.001).Additionally,98 cases were positive only by liquid culture method,representing a positive rate of 26.63%,while 80 cases were positive only by PCR method,with a positive rate of 21.74%.The consistency of detection results between the two methods was moderate(Kappa=0.734).The majority of patients who tested positive for Uu by both methods were concentrated in the 20～39 age group.Among 58 cases with isolated Uu positivity,the susceptibility rates to mi-nocycline and josamycin were both 100%,and the susceptibility rates to doxycycline and clar-ithromycin were all above 90%.Conclusions There are differences in the positive rates of Uu de-tection between fluorescence quantitative PCR and liquid culture methods.Combined detection u-sing both methods should be considered when necessary to enhance diagnostic accuracy.In clini-cal practice,minocycline,josamycin,doxycycline,and clarithromycin,which demonstrate high susceptibility,can be recommended as first-line treatments for Uu-positive patients.
代红丹;李红林;王希
重庆市中医院,重庆 400011重庆市中医院,重庆 400011重庆市中医院,重庆 400011
解脲脲原体荧光定量PCR液体培养法
Ureaplasma urealyticumfluorescence quantitative PCRliquid culture method
《皮肤性病诊疗学杂志》 2026 (1)
16-20,5
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