蓝光联合凝胶治疗皮肤光老化模型小鼠的效果和作用机制OA
Therapeutic effects and mechanisms of blue light combined with gel for skin photoaging in mice
目的 研究蓝光联合凝胶技术对皮肤光老化模型小鼠的治疗效果和作用机制.方法 将25只雄性C57/BL6 小鼠随机分为5 组:空白对照组、模型组、蓝光组、凝胶组、蓝光+凝胶(联合)组,每组5 只.除空白对照组外,其它组别小鼠采取UVA和UVB协同辐照的造模方法构建皮肤光老化模型:从第 1 周到第4 周采取 1 个最小红斑量(MED)的剂量,第5 周和第6 周采取2 个MED的剂量进行照射;同时,从第3 周开始,每周的最后一天,凝胶组采用凝胶处理 6 min,蓝光组采用蓝光照射6 min,联合组采用凝胶处理后立即用蓝光照射6 min,空白对照组和模型组不做处理.6 周后,取背部皮肤进行HE和Masson染色,同时采用ELISA方法测定皮肤和血清中超氧化歧化酶(SOD)、谷胱甘肽(GSH-Px)和肿瘤坏死因子(TNF-α)等因子水平.结果 第6 周后:模型组小鼠皮肤表面出现脱屑、干燥、粗糙、皮损、皮革状、色沉等;与模型组相比,蓝光组或凝胶组皮肤脱屑、干燥、粗糙和皮革状未得到显著改善,SOD和GSH-Px的表达无显著性上调(均P＞0.05),胶原表达未显著增加(均P＞0.05);联合组皮肤皱纹、干燥、脱屑和皮革状有显著性改善,皮肤中 SOD 和 GSH-Px 等表达显著上调并恢复到正常水平(P值分别为0.028、0.043),胶原含量显著增加(P＜0.001),TNF-α的表达无明显变化(P＞0.05),血清中SOD、GSH-Px和TNF-α的水平均无明显变化(P＞0.05).结论 蓝光联合凝胶可抗氧化、降低光老化皮肤炎症水平、逆转光老化胶原蛋白的流失,为皮肤光老化的临床应用提供了实验依据.
Objective To investigate the therapeutic effects and mechanisms of blue light com-bined with gel for skin photoaging in mice.Methods Twenty-five male C57/BL6 mice were ran-domly divided into five groups:blank control,model,blue light,gel,and blue light+gel(com-bination)groups,with five mice per group.Except for the blank control group,a UVA and UVB synergistic irradiation method was used to establish a photoaging model.Mice were irradiated with 1 minimal erythema dose(MED)from week 1 to week 4,followed by 2 MEDs in weeks 5 and 6.Concurrently,starting from the third week,blue light combined with gel therapy was administered on the last day of each week.The control group received no treatment.The gel group received on-ly gel treatment for 6 minutes.The blue light group received only blue light irradiation for 6 mi-nutes.The combined group received gel treatment,followed immediately by blue light therapy.After 6 weeks,dorsal skin samples were collected for hematoxylin and eosin(HE),and Masson＇s trichrome staining.Both cutaneous and serum levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and tumor necrosis factor(TNF-α)were measured using ELISA.Results After 6 weeks:The mouse skin exhibited desquamation,dryness,roughness,lesions,leathery texture,and hyperpigmentation.Either the blue light or gel group showed no significant improve-ments in skin desquamation,dryness,roughness,or leathery texture compared with the model group.Moreover,expression levels of SOD,GSH-Px,and collagen were not significantly upregu-lated compared with the model group(P＞0.05).However,the combined group showed signifi-cant improvement in wrinkles,dryness,desquamation,and leathery texture.The expression levels of SOD and GSH-Px in the skin were significantly upregulated to levels comparable to normal(P＜0.05),accompanied by a significant increase in collagen(P＜0.001).TNF-αexpression in the skin,as well as the serum levels of SOD,GSH-Px,and TNF-α,remained no significant change(P＞0.05).Conclusions Blue light combined with gel can alleviate oxidation,reduce inflammation,and increase collagen in photoaged skin,providing experimental evidence for its clinical application in photoaging management.
王著显;陈秋禹;周思洁;王炫;刘军;杨斌
南方医科大学皮肤病医院,广东 广州 510091南方医科大学皮肤病医院,广东 广州 510091南方医科大学皮肤病医院,广东 广州 510091南方医科大学皮肤病医院,广东 广州 510091南方医科大学皮肤病医院,广东 广州 510091南方医科大学皮肤病医院,广东 广州 510091
皮肤光老化蓝光凝胶超氧化歧化酶胶原
skin photoagingblue lighthydrogelsuperoxide dismutasecollagen
《皮肤性病诊疗学杂志》 2026 (1)
1-8,8
广东省自然科学基金(2025A1515010823)
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