基于斑马鱼模型对Betukladin降血脂活性与机制分析OA

Betukladin is a dietary supplement composed of reindeer lichen powder and birch bark extract,possessing various pharmacological activities such as blood glucose regulation,lipid lowering,anti-inflammatory effects,and anticoagulation.This study investigates the lipid-lowering activity and underlying mechanism of Betukladin,aiming to validate its efficacy and provide a scientific basis for developing safe and economical natural lipid-lowering drugs.Using a zebrafish hyperlipidemia model,we found that Betukladin significantly improved the elevated Oil Red O staining integrated optical density values,triglyceride levels,and total cholesterol levels induced by high-fat modeling,indicating its lipid-lowering activity.Network pharmacology analysis suggested that Betukladin may exert its lipid-lowering effects through multiple coordinated pathways,including lipid and atherosclerosis pathways and the PPAR signaling pathway.RT-qPCR results showed that it can regulate lipid metabolism-related genes(apoA1,LDLR,and ANGPTL3),fatty acid synthesis and oxidation genes(SREBF1,FASN,PPARα,and cpt1a,),and cholesterol metabolism-related genes(CYP7A1,HMGCR,ABCG5,and ABCG8).