Ligilactobacillus cholophilus BD7642全基因组分析及功能预测OA
Whole Genome Analysis and Functional Prediction of Ligilactobacillus cholophilus BD7642
从上海发酵雪菜中分离获得一株新菌株Ligilactobacillus cholophilus BD7642,通过二代+三代测序方式获得了该菌株的全基因组序列信息,为对该菌株进行表征,并发掘其潜在的益生功能,对获得的基因组序列展开分析.结果表明,该菌株基因组大小为1 587 935 bp,编码1 530 个基因,没有质粒存在.对其进行功能注释、次级代谢产物合成分析,发现其具有细菌素Mutacin 1140和III型聚酮化合物合酶基因簇,功能注释揭示该菌株具有强大的环境适应能力,ResFinder软件预测其无耐药基因,比较基因组学分析进一步揭示了该菌株的基因组特异性.本研究结果可为进一步了解L.cholophilus BD7642、挖掘其益生功能提供参考.
A novel strain of Ligilactobacillus cholophilus,designated BD7642,was isolated from pickled potherb mustard(Brassica juncea Coss.)in Shanghai.The complete genome sequence of the strain was obtained using a combination of PacBio and Illumina sequencing.To characterize this strain and explore its potential probiotic functions,we performed comprehensive genomic analyses.The results showed that the genome of L.cholophilus BD7642 was 1 587 935 bp in size and encoded 1 530 genes(no plasmids detected).Functional annotation and secondary metabolite synthesis analysis revealed that L.cholophilus BD7642 contained gene clusters for the biosynthesis of the bacteriocin mutacin 1140 and type III polyketide synthases and possessed a great capacity for environmental adaptation.ResFinder analysis indicated the absence of antibiotic resistance genes.Comparative genomics uncovered the genomic uniqueness of L.cholophilus BD7642.This study provides a deep understanding of L.cholophilus BD7642 and offers a reference for exploiting its probiotic potential.
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乳业生物技术国家重点实验室,上海乳业生物工程技术研究中心,光明乳业股份有限公司,上海 201103
轻工纺织
Ligilactobacillus cholophilus基因组学生物信息学细菌素
Ligilactobacillus cholophilusgenomicsbioinformaticsbacteriocin
《乳业科学与技术》 2026 (1)
16-22,7
上海市国资委企业创新发展和能级提升项目(2022013)"十四五"国家重点研发计划重点专项(2022YFD2100704)
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