超声优化miR-26a脂质体与牛乳外泌体的递送效能对比分析OA

Based on previous research suggesting that milk exosomes specifically express miR-26a,this study optimized the miR-26a encapsulation efficiency of liposomes through ultrasonic treatment,and it also evaluated the in vitro digestion performance of the ultrasonic-treated liposomes and compared its anti-inflammatory activity with that of natural milk exosomes.Single-factor experiments were conducted to optimize the molar charge ratio of cationic liposome vector to nucleic acid(N/P),ultrasonication time,and ultrasonic power for the preparation of miR-26a-encapsulated liposomes.The results demonstrated that when the N/P ratio was 4,ultrasonication time was 15 min,and ultrasonic power was 300 W,the encapsulation efficiency reached its peak of(89.51±1.37)%,with uniform particle size distribution and homogeneous morphology.In vitro simulated digestion experiments showed that the retention rate of miR-26a in the ultrasonic-treated liposomes(58%)was higher than that in the conventional liposomes(24%).Cell function experiments confirmed that the ultrasonic-treated liposomes not only enhanced the viability of normal Caco-2 cells but also effectively inhibited lipopolysaccharide(LPS)-induced inflammatory responses,significantly restoring cell viability under inflammatory conditions.Additionally,it reduced NO release and down-regulated the secretion of the inflammatory cytokines interleukin 6(IL-6),tumor necrosis factor α,and IL-1β,while effectively controlling the generation of reactive oxygen species.Notably,the ultrasonic-treated liposomes showed no significant differences from milk exosomes in terms of cell viability regulatory and inflammation inhibitory effects.In summary,this study demonstrates that ultrasonic can significantly improve the miR-26a delivery efficiency of liposomes.