烟酰胺核苷对微波辐射致小鼠脑损伤的影响研究OA

Objective To explore the neuroprotective effects of nicotinamide riboside against microwave radiation-induced brain injury in mice.Methods Mice were subjected to whole-body uniform microwave radiation(at a central frequency of 2.856 GHz,and average power density of 20 mW/cm² for 30 min).After radiation,the mice received intraperitoneal injections of nicotinamide riboside chloride at doses of 250,500,or 1000 mg/(kg·d).Behavioral performance was assessed using the open field test(OFT)and elevated plus maze(EPM).Histopathological changes in the cortex(CTX),hippocampus(HPC),and hypothalamus(HT)were detected via hematoxylin-eosin(HE)staining.Levels of such pro-inflammatory cytokines as tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in the CTX,HPC,and HT were measured using enzyme-linked immunosorbent assay(ELISA).Proteomic analysis was performed to assess changes in protein expressions in the CTX.Results After microwave exposure,the exploratory behavior of mice was reduced,and the entries into the central zone and open arms were decreased(P<0.05).Neuronal injuries were increased,and the levels of TNF-α and IL-6 were elevated(P<0.05).Proteomic analysis indicated that differentially expressed proteins were mostly enriched through oxidative phosphorylation related pathways.NR interventions significantly ameliorated these alterations,as evidenced by increased exploratory behavior,reduced neuronal injury,decreased inflammatory cytokine levels(P<0.05),and the reversal of expressions of dysregulated proteins,including zinc finger AN1-type domain-containing protein 5(Zfand5).Conclusion NR can alleviate brain injury and associated behavioral and neuroinflammatory alterations in mice induced by microwave radiation,which might involve the zfands and other key molecules.