基于生物信息学分析苍术抑菌作用研究OA

Objective To clarify the main antibacterial parts of Cangzhu(Atractylodis Rhizoma)and its antibacterial effects on various bacteria.Methods The components,targets and antibacterial targets of Cangzhu(Atractylodis Rhizoma)were predicted and screened by database.DAVID database was used for gene ontology(GO)function enrichment analysis and Kyoto Gene and Genome Database(KEGG)pathway enrichment analysis.Cytoscape 3.10.2 software is used to map"disease-pathway-target-ingredient-drug".Using Autodock software for molecular docking;Staphylococcus aureus,Enterococcus faecalis,Pseudomonas aeruginosa,Acinetobacter baumannii,Staphylococcus epidermidis,Propionibacterium acnes and Malazzae furfur were tested in vitro to evaluate the inhibitory effect.Results A total of 34 active components,355 targets,3678 antibacterial targets and 200 potential therapeutic targets were obtained through network pharmacological screening.Potential antibacterial components and core targets have good binding ability,among which the top-ranked components are all effective components of volatile oil,so it is speculated that volatile oil is the main part of Cangzhu(Atractylodis Rhizoma)to exert antibacterial effect,and verified by in vitro experiments.Staphylococcus aureus,Enterococcus faecalis,Propionibacterium acens and Malassezia furfur were moderately sensitive to the volatile oil of Cangzhu(Atractylodis Rhizoma),and were low sensitive to Staphylococcus epidermidis.It had no inhibitory effect on Pseudomonas aeruginosa and Acinetobacter baumannii.Conclusion The results of network pharmacology and molecular docking technology showed that the main antibacterial part of Cangzhu(Atractylodis Rhizoma)was volatile oil.In vitro antibacterial experiment showed that the essential oil of Cangzhu(Atractylodis Rhizoma)had good antibacterial effect,among which the inhibitory effect on Malassezia furfur was the strongest,Staphylococcus aureus and Propionibacterium acnes were stronger.The inhibitory effect on Staphylococcus epidermidis was weak.It had no inhibitory effect on Pseudomonas aeruginosa and Acinetobacter baumannii.