炎性细胞因子、MAOB基因13内含子G/A、SNCA基因rs3822086位点多态性与帕金森病的相关性研究OA

Objective To explore the correlation between IL-related inflammatory factor expression,MAOB gene 13 intron G/A(MAOB-13G/A)polymorphism,SNCA gene rs3822086 locus polymorphism and Parkinson's disease(PD).Methods A total of 94 PD patients and 110 healthy controls were enrolled in this study.The levels of IL-1α,IL-6,IL-8 and IL-10 were determined by ELISA,and the genotypes of MAOB-13G/A and SNCA-rs3822086 locus polymorphisms were detected by restriction fragment length polymorphism polymerase chain reaction(PCR-RFLP).The levels of IL-related inflammatory factors in patients with different MAOB and SNCA genotypes were compared between the two groups.Results There was no significant difference in genotype distribution of MAOB-13G/A and SNCA-rs3822086 locus genotypes between PD group and normal control group(all P>0.05).There were significant differences in serum IL-6 levels among the early stage group,the middle stage group and the late stage group(P<0.05),while there was no statistically significant difference in serumIL-1α,IL-8,and IL-10 levels(all P>0.05).The serum levels of IL-1α,IL-8 and IL-10 in PD group were significantly higher than those in normal control group(all P<0.05),while there was no statistically significant difference in serum IL-6 level(P>0.05).Compared with those in normal control group,the serum levels of IL-1α,IL-8 and IL-10 in PD group with MAOB G/G and A/A genotypes were significantly increased(all P<0.05),while there was no statistically significant difference in serum IL-6 levels(P>0.05);the serum levels of IL-8 and IL-10 in PD group with MAOB G/A genotype were significantly increased(all P<0.05),while there was no statistically significant difference in serum IL-1α and IL-6 levels(all P>0.05).Compared with those in normal control group,the serum levels of IL-8 and IL-10 in PD group with SNCA C/C and C/T genotypes were significantly increased(all P<0.05),while there was no statistically significant difference in serum IL-1α and IL-6 levels(all P>0.05);the serum levels of IL-1α and IL-8 in PD group with T/T genotype were significantly increased(all P<0.05),while the serum IL-6 level was significantly decreased(P<0.05),and there was no statistically significant difference in serum IL-10 levels(P>0.05).Conclusions The increase in serum IL-6 level is related to the aggravation of PD,and the increase in serum IL-1α,IL-8,and IL-10 expression levels may be risk factors for PD.The different genotypes of MAOB-13G/A and SNCA-rs3822086 loci can change the levels of IL-1α and IL-10,thereby affecting the progression of PD.The SNCA T/T genotype may be a genetic factor causing low IL-6 level.