施肥处理下福建柏叶精油成分及其抑菌活性研究OA

This study investigated the effects of N 300 g,P 200 g,K 50 g,Mg 18.2 g fertilization treatments on the chemical composition and antimicrobial activity of the essential oil extracted from the leaves of 6-year-old Chamaecyparis hodginsii.The essential oil was obtained by hydrodistillation,and its chemical composition was analyzed using GC-MS.The in vitro antimicrobial activity was evaluated against 3 common foodborne pathogenic bacteria(Staphylococcus aureus,Escherichia coli,and Bacillus subtilis),and 3 pathogenic fungi(Aspergillus flavus,Aspergillus niger,and Penicillium citrinum).The results showed that 52 compounds were identified from the essential oil after 2 fertilization treatments.Among them,there were 15 main components with a GC content greater than 1％,and the total GC content was94.10％,with α-pinene(51.51％)and D-limonene(9.19％)being the major constituents.The essential oil exhibited significant and broad-spectrum antimicrobial activity against all tested strains.It was highly sensitive to S.aureus and A.flavus,moderately sensitive to E.coli,B.subtilis,and P.citrinum,and less sensitive to A.niger.The minimum inhibitory volume fraction(MIC)values for S.aureus,E.coli,B.subtilis,A.flavus,A.niger,and P.citrinum were 5％,10％,2.5％,2.5％,10％,and 5％,respectively.The minimum bactericidal fungicidal volume fraction(MBC)values were 10％,10％,5％,5％,10％,and 10％,respectively.In conclusion,the essential oil from C.hodginsii leaves demonstrates notable broad-spectrum antimicrobial activity,which is influenced by fertilization treatment.