生物炭固定化木霉菌剂制备及其对聚乙烯的降解OA

In order to improve the degradation efficiency of polyethylene,using biochar as the carrier and Trichoderma as the strain,the biochar immobilized Trichoderma was prepared by adsorption method.Suitable biochar carriers were selected from biochars prepared at four different pyrolysis temperatures,and the preparation conditions of immobilization temperature,inoculation amounts and immobilization medium were optimized to evaluate the degradation effect of immobilized microbial agents on polyethylene microplastics.The results show that 300℃ biochar BC300 has abundant functional groups and better biomass of immobilized microbial agents.The degradation effect of polyethylene microplastics by biochar immobilized Trichoderma FBC300 is better than that of the other three immobilized microbial agents,then BC300 is selected as the carrier.The single factor optimization test shows that the conditions of 20 ℃ immobilization temperature,5％(mass fraction)inoculation amount,the potato glucose liquid medium are the best.In the simulation application,the polyethylene degradation rate of FBC300 is 0.854％ after 14 days of co-culture,and the degradation effect is better than that of free Trichoderma and biochar,indicating that the immobilized Trichoderma FBC300 can be used as a good material to reduce the pollution of polyethylene microplastics.