基于生理响应与转录组分析β-氨基丁酸增强水稻幼苗抗冷性OA
Analysis of β-aminobutyric acid enhancing cold resistance of rice seedlings based on physiological responses and transcriptome analysis
[目的]早稻秧苗期易遭受低温胁迫,旨在探究广谱化学诱导剂β-氨基丁酸(BABA)对低温胁迫下水稻幼苗生理特性及分子机制的调控作用.[方法]以0.04～1 mmol/L BABA灌根处理粳稻中花11幼苗2 d后,对照(CK)为清水处理,置于冷胁迫(8℃)下7 d,考查常温恢复生长7 d后的幼苗生长状况,测定冷胁迫0～6 d抗氧化相关酶活性和抗性相关物质含量的变化,并进行转录组测序.[结果]与CK相比,0.2 mmol/L BABA能显著促进冷害后水稻幼苗的恢复生长,冷胁迫1 d后SOD、CAT、APX、GR、DHAR活性升高,H2O2、MDA和Pro含量降低,AsA和GSH含量升高.比较BABA处理与CK在冷胁迫6 h、3 d和6 d的转录组,共获得2 616个差异表达基因,其中1 053个基因上调,1 563个基因下调.GO生物过程功能分析显示,差异表达基因富集于胁迫响应、二萜生物合成、碳水化合物代谢、氧化胁迫响应等条目.KEGG通路分析发现,差异表达基因在次生代谢物合成、苯丙素生物合成、植物激素信号转导、MAPK通路上有显著富集.进一步分析激素信号相关的差异基因发现,冷胁迫下BABA能在转录水平上激活ABA信号,抑制JA、SA信号.[结论]BABA能增强冷胁迫下幼苗的氧化代谢能力,调控胁迫响应、激素信号、次生代谢物合成通路上相关基因的表达,从而提高水稻幼苗的抗冷性.
[Objective]Early rice seedlings are prone to low-temperature stress.This study explores the regulation of the physiological characteristics and molecular mechanisms of rice seedlings under low-temperature stress by the broad-spectrum chemical inducer β-aminobutyric acid(BABA).[Method]Two-day-old rice seedlings at the panicle differentiation stage 11 were treated with 0.04-1 mmol/L BABA via root irrigation,with water treatment as the control(CK).The seedlings were subjected to cold stress(8℃)for 7 days,and the seedling growth was assessed after 7 days of recovery at normal temperature.Changes in antioxidant-related enzyme activities and resistance-related substance contents from 0 to 6 days of cold stress were measured,and transcriptome sequencing was performed.[Result]Compared with CK,0.2 mmol/L BABA can significantly promote the recovery growth of rice seedlings after cold damage,increase the activities of SOD,CAT,APX,GR,and DHAR one day after cold stress,reduce the contents of H2O2,MDA,and Pro,and increase the contents of AsA and GSH.Comparing the transcriptomes of BABA treatment and CK under cold stress for 6 hours,3 days,and 6 days,a total of 2 616 differentially expressed genes were identified,of which 1 053 genes were upregulated and 1 563 genes were downregulated.GO biological process analysis showed that the differentially expressed genes were enriched in stress response,diterpenoid biosynthesis,carbohydrate metabolism,oxidative stress response,and other categories.KEGG pathway analysis revealed that the differentially expressed genes were significantly enriched in secondary metabolite biosynthesis,phenylpropanoid biosynthesis,plant hormone signal transduction,and MAPK pathways.Further analysis of hormone-related differentially expressed genes showed that under cold stress,BABA can transcriptionally activate ABA signaling while inhibiting JA and SA signaling.[Conclusion]BABA can enhance the antioxidant capacity of seedlings under cold stress,and regulate the expression of genes related to stress response,hormone signaling,and secondary metabolite synthesis pathways,thereby improving the cold tolerance of rice seedlings.
陈高付;张海洋;刘小慧;王梦醒;何永明
江西农业大学 农学院/作物生理生态与遗传育种教育部重点实验室,江西 南昌 330045江西农业大学 农学院/作物生理生态与遗传育种教育部重点实验室,江西 南昌 330045江西农业大学 农学院/作物生理生态与遗传育种教育部重点实验室,江西 南昌 330045江西农业大学 农学院/作物生理生态与遗传育种教育部重点实验室,江西 南昌 330045江西农业大学 农学院/作物生理生态与遗传育种教育部重点实验室,江西 南昌 330045
农业科技
水稻β-氨基丁酸冷胁迫氧化代谢转录组
riceβ-Aminobutyric acidcold stressoxidative metabolismtranscriptome
《江西农业大学学报》 2026 (1)
11-22,12
国家自然科学基金项目(31360295)和江西省自然科学基金项目(20212BAB205006) Projects supported by the National Natural Science Foundation of China(31360295)and Jiangxi Provincial Natu-ral Science Foundation(20212BAB205006)
评论