HIV-1假病毒标准物质的制备及应用研究OA

Based on lentiviral packaging system,the co-transfection technology of multi-plasmid was used to deliver the relevant genes into host cells containing specific nucleic acid sequences,and the human immunodeficiency virus(HIV-1)pseudovirus reference materials were fabricated.The corresponding spike protein was expressed on the outer shell.The standard value of copy number and expanded uncertainly of the reference materials was(300.12±0.54)×104 copies/mL(k=2)by digital polymerase chain reaction(PCR).Taking advantage of the characteristic that the pseudovirus infects by the recognition between the spike protein and the receptor of host cell,the binding could be interfered by adding neutralizing antibodies to inhibit the infection of the pseudovirus.By observing the inhibition degree of neutralizing antibodies at different concentrations on the expression of fluorescence,the Reed-Muench two-formula method was used to calculate the ZPD50 and evaluated the potency of neutralizing antibodies.