半乳糖凝集素-9和音猬因子表达检测在结直肠癌早期诊断及预后中的作用OA
Role of Galectin-9 and Sonic Hedgehog Expressions in the Early Diagnosis and Prognosis of Colorectal Cancer
目的 观察结直肠癌患者癌组织中半乳糖凝集素-9(galectin-9)和音猬因子(sonic hedgehog,SHH)的表达变化,分析二者与结直肠癌发生风险及预后的关联.方法 回顾性纳入2020年5月-2022年5月青岛市中医医院收治的结直肠癌患者210例,检测其癌组织及癌旁组织中galectin-9与SHH的表达水平.采用logistic回归分析两者表达与结直肠癌发病风险之间的关系,并绘制受试者工作特征(receiver operating characteristic,ROC)曲线,评估二者对结直肠癌发病风险及预后的预测价值.结果 与癌旁组织相比,癌组织中galectin-9和SHH的表达水平差异均有统计学意义(P<0.05).ROC曲线分析显示,galectin-9和SHH表达水平评估结直肠癌发生风险的曲线下面积(area under the curve,AUC)分别为0.809(95%CI:0.751～0.868)和0.865(95%CI:0.817～0.912).在T3-4、N1-3、M1及低分化患者中galectin-9表达降低(P<0.05),而SHH表达水平则升高(P<0.05).Galectin-9与SHH表达及TNM分期呈负相关(r=-0.184、-0.362,P<0.001);SHH表达与TNM分期正相关(r=0.407,P<0.001).预后不良与预后良好患者galectin-9、SHH表达水平比较差异有统计学意义(P<0.05).Logistic回归分析显示,T3-4期(OR=4.609,95%CI:1.461～14.535)、低分化(OR=3.337,95%CI:1.297～8.582)及SHH(OR=2.067,95%CI:1.162～3.678)水平为影响患者预后的危险因素(P<0.05),galectin-9(OR=0.652,95%CI:0.437～0.975)为影响患者预后的保护因素(P<0.05).ROC曲线显示,galectin-9、SHH表达水平评估结直肠癌预后的AUC分别为0.769(95%CI:0.550～0.798)和0.734(95%CI:0.603～0.866).结论 Galectin-9、SHH在结直肠癌患者中分别呈低表达和高表达,其表达水平与肿瘤的低分化、侵袭和转移行为有关,且对患者患病风险和预后具有一定的评估价值.
Objective To investigate the association between the expression levels of galectin-9 and sonic hedgehog(SHH)and the risk of occurrence and prognosis in patients with colorectal cancer(CRC).Methods A total of 210 patients with CRC treated at Qingdao Traditional Chinese Medicine Hospital between May 2020 and May 2022 were retrospectively included.The expressions of galectin-9 and SHH in tumor tissues were measured.Logistic regression was used to analyze the relationship between galectin-9/SHH expression and CRC risk.Receiver operating characteristic(ROC)curves were plotted to evaluate the predictive value of galectin-9 and SHH for CRC risk and prognosis.Results The expression levels of galectin-9 and SHH differed significantly between cancer tissues and adjacent normal tissues(P<0.05).ROC analysis showed that the AUCs for galectin-9 and SHH in evaluating CRC risk were 0.809(95%CI:0.751-0.868)and 0.865(95%CI:0.817-0.912),respectively.Galectin-9 expression was lower in patients with T3-4 stage,N1-3 stage,M1 stage,or poor differentiation(P<0.05),whereas SHH expression was higher in these patients(P<0.05).Galectin-9 expression was negatively correlated with SHH expression and TNM stage(r=-0.184 and-0.362,respectively;P<0.001).SHH expression was positively correlated with TNM stage(r=0.407,P<0.001).Significant differences in galectin-9 and SHH expression were observed between patients with poor prognosis and those with good prognosis(P<0.05).Logistic regression analysis indicated that T3-4 stage(OR=4.609,95%CI:1.461-14.535),poor differentiation(OR=3.337,95%CI:1.297-8.582),and high SHH expression(OR=2.067,95%CI:1.162-3.678)were risk factors for poor prognosis(P<0.05),while high galectin-9 expression(OR=0.652,95%CI:0.437-0.975)was a protective factor(P<0.05).The AUCs of galectin-9 and SHH for evaluating CRC prognosis were 0.769(95%CI:0.550-0.798)and 0.734(95%CI:0.603-0.866),respectively.Conclusion Galectin-9 and SHH are lowly and highly expressed,respectively,in CRC.Their expression levels are associated with tumor differentiation,invasion,and metastasis,and hold predictive value for the risk and prognosis of CRC patients.
何倩;刘昕;林甜甜;董笑楠
青岛大学附属青岛市海慈医院(青岛市中医医院)肛肠科(青岛 266000)青岛大学附属青岛市海慈医院(青岛市中医医院)肛肠科(青岛 266000)青岛大学附属青岛市海慈医院(青岛市中医医院)普外科(青岛 266000)青岛大学附属青岛市海慈医院(青岛市中医医院)肛肠科(青岛 266000)
结直肠癌半乳糖凝集素-9音猬因子预后预测价值
Colorectal cancerGalectin-9Sonic hedgehogPrognosisPredictive value
《四川大学学报（医学版）》 2026 (1)
217-223,7
This study was supported by 2022 Shandong Provincial Traditional Chinese Medicine Science and Technology Project(No.Q-2022009). 2022年度山东省中医药科技项目(No.Q-2022009)
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