山西省玉米大斑病菌生理小种、交配型及遗传多样性分析OA

To clarify the physiological races,mating type composition,and genetic diversity of Exserohilum turcicum in Shanxi Province,the Ht single gene differential hosts were employed to determine the physiological races of the tested isolates,the mating type specific primers were utilized to determine their mating types,and genetic diversity was analyzed using SSR molecular markers in this study.The results showed that 16 physiological races of E.turcicum(123N,123,23N,13N,12N,3N,2N,1N,23,13,12,N,3,2,1,0)were identified in Shanxi Province,with 123N and 13N being the dominant races.Three mating types of Aa,A,and a were detected,with a being the most prevalent.SSR analysis of 52 E.turcicum isolates using 6 SSR primers yielded 40 polymorphic bands,achieving a polymorphism rate of 100％.Based on a genetic similarity coefficient of 0.69,the tested isolates were clustered into 10 distinct groups.Molecular variance analysis revealed that SSR-based classification exhibited no strong correlation with geographical origin or physiological race but showed a significant association with mating types.These findings provide valuable insights into the genetic diversity of E.turcicum in Shanxi Province and offer a theoretical foundation for effective management of northern corn leaf blight infected by Exserohilum turcicum.